Flow injection analysis of carbofuran in foods using air stable lipid film based acetylcholinesterase biosensor

Dimitrios P. Nikolelis, Maria G. Simantiraki, Christina G. Siontorou, K. Tóth

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

This work describes a technique for the rapid, selective and sensitive electrochemical flow injection analysis of carbofuran in foods using air stable lipid films in which acetylcholinesterase was incorporated. These lipid films were supported on a methylacrylate polymer (i.e., methacrylic acid was the functional monomer for the polymerization, ethylene glycol dimethacrylate was used as the crosslinker and 2,2′-azobis-2-methylpropionitrile as an initiator). The substrate was injected into flowing streams of a carrier electrolyte solution. Hydronium ions produced by the enzymatic reaction at the lipid film surface caused dynamic alterations of the electrostatic fields and phase structure of membranes and as a result ion current transients were obtained; the magnitude of these signals was correlated to the substrate concentration, which could be determined at the micromolar level. The response times were ca. 10 s. These lipid membranes were used for the flow injection analysis of pesticides. The principle of the method is based on the degree of inhibition of the enzyme by the pesticide. Carbofuran was chosen as a typical pesticide. The novelty of the method is based on the fact that an "air segmented" flow injection technique can be used as compared to previous systems that are based on lipid film technology but are not stable in air. This allows determination of the pesticide using the degree of inhibition and reactivation of enzyme by injections of substrate. Carbofuran was determined at concentration levels of 10-7 to 10-9 M. The investigation of the effect of potent interferences included a wide range of compounds usually found in foods and also proteins and lipids. The technique was applied in 50 different real samples of fruits, vegetables and dairy products. The recovery ranged between ca. 96 and 106% which shows no interferences from the matrix effects.

Original languageEnglish
Pages (from-to)169-177
Number of pages9
JournalAnalytica Chimica Acta
Volume537
Issue number1-2
DOIs
Publication statusPublished - Apr 29 2005

Fingerprint

Carbofuran
Flow Injection Analysis
carbofuran
Biosensing Techniques
Acetylcholinesterase
Biosensors
Pesticides
lipid
Air
Lipids
Food
food
air
pesticide
Polymethacrylic Acids
substrate
Substrates
Dairy products
Injections
Dairy Products

Keywords

  • Acetylcholinesterase
  • Air stable lipid film
  • Carbofuran
  • Foods

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Flow injection analysis of carbofuran in foods using air stable lipid film based acetylcholinesterase biosensor. / Nikolelis, Dimitrios P.; Simantiraki, Maria G.; Siontorou, Christina G.; Tóth, K.

In: Analytica Chimica Acta, Vol. 537, No. 1-2, 29.04.2005, p. 169-177.

Research output: Contribution to journalArticle

Nikolelis, Dimitrios P. ; Simantiraki, Maria G. ; Siontorou, Christina G. ; Tóth, K. / Flow injection analysis of carbofuran in foods using air stable lipid film based acetylcholinesterase biosensor. In: Analytica Chimica Acta. 2005 ; Vol. 537, No. 1-2. pp. 169-177.
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