Flow immunoassay using solid-phase entrapment

Laurie Locascio-Brown, Larissa Martynova, Richard G. Christensen, George Horvai

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A flow injection immunoassay was performed using a column packed with reversed-phase sorbents to effect separation of the immunoreacted species by entrapping free analyte and allowing antibody-conjugated analyte to pass unretained. Fluorescein-labeled analyte was measured in a competitive assay for the anticonvulsant drug phenytoin. The simplicity of the assay was the greatest advantage of the technique, which allowed for measurement of phenytoin in a 2-min assay time. The reliable detection limit for the assay was 5 nmol L-1 of phenytoin in serum. The columns were regenerated with periodic injections of ethanol solutions to remove the entrapped analyte and prepare the column for subsequent analyses.

Original languageEnglish
Pages (from-to)1665-1670
Number of pages6
JournalAnalytical Chemistry
Issue number9
Publication statusPublished - Jan 1 1996


ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Locascio-Brown, L., Martynova, L., Christensen, R. G., & Horvai, G. (1996). Flow immunoassay using solid-phase entrapment. Analytical Chemistry, 68(9), 1665-1670. https://doi.org/10.1021/ac951173n