Flow immunoassay using solid-phase entrapment

Laurie Locascio-Brown, Larissa Martynova, Richard G. Christensen, G. Horvai

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A flow injection immunoassay was performed using a column packed with reversed-phase sorbents to effect separation of the immunoreacted species by entrapping free analyte and allowing antibody-conjugated analyte to pass unretained. Fluorescein-labeled analyte was measured in a competitive assay for the anticonvulsant drug phenytoin. The simplicity of the assay was the greatest advantage of the technique, which allowed for measurement of phenytoin in a 2-min assay time. The reliable detection limit for the assay was 5 nmol L-1 of phenytoin in serum. The columns were regenerated with periodic injections of ethanol solutions to remove the entrapped analyte and prepare the column for subsequent analyses.

Original languageEnglish
Pages (from-to)1665-1670
Number of pages6
JournalAnalytical Chemistry
Volume68
Issue number9
Publication statusPublished - 1996

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Assays
Phenytoin
Sorbents
Fluorescein
Anticonvulsants
Ethanol
Antibodies

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Locascio-Brown, L., Martynova, L., Christensen, R. G., & Horvai, G. (1996). Flow immunoassay using solid-phase entrapment. Analytical Chemistry, 68(9), 1665-1670.

Flow immunoassay using solid-phase entrapment. / Locascio-Brown, Laurie; Martynova, Larissa; Christensen, Richard G.; Horvai, G.

In: Analytical Chemistry, Vol. 68, No. 9, 1996, p. 1665-1670.

Research output: Contribution to journalArticle

Locascio-Brown, L, Martynova, L, Christensen, RG & Horvai, G 1996, 'Flow immunoassay using solid-phase entrapment', Analytical Chemistry, vol. 68, no. 9, pp. 1665-1670.
Locascio-Brown L, Martynova L, Christensen RG, Horvai G. Flow immunoassay using solid-phase entrapment. Analytical Chemistry. 1996;68(9):1665-1670.
Locascio-Brown, Laurie ; Martynova, Larissa ; Christensen, Richard G. ; Horvai, G. / Flow immunoassay using solid-phase entrapment. In: Analytical Chemistry. 1996 ; Vol. 68, No. 9. pp. 1665-1670.
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