Flow cytometric measurements and electron microscopy of cell surface glycosaminoglycans using Acridine Orange

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Abstract

Cell surface glycosaminoglycans (GAGs) were measured, after various treatments, by their binding to Acridine Organge using flow cytometry. Using a critical electrolyte concentration and combining it with specific degradation of individual GAG elements, it was found possible to differentiate between GAG components. The technique was adapted for electron microscopy level to reveal characteristics of membrane-associated GAG. By this means, the cell membrane of the human leukaemic cell line K562 was shown to contain a large amount of GAG; 75% of it was highly sulphated GAG, mostly heparan sulphate. This component was evenly distributed in the outer plasma membrane layer. In the presence of other GAGs, the appearance of complex proteoglycan granules was detected.

Original languageEnglish
Pages (from-to)71-79
Number of pages9
JournalThe Histochemical Journal
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 1 1985

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ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

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