Flow cytometric and laser scanning microscopic approaches in epigenetics research

Lorant Szekvolgyi, Laszlo Imre, Doan Xuan Quang Minh, Eva Hegedus, Zsolt Bacso, Gabor Szabo

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Our understanding of epigenetics has been transformed in recent years by the advance of technological possibilities based primarily on a powerful tool, chromatin immunoprecipitation (ChIP). However, in many cases, the detection of epigenetic changes requires methods providing a high-throughput (HTP) platform. Cytometry has opened a novel approach for the quantitative measurement of molecules, including PCR products, anchored to appropriately addressed microbeads (Pataki et al. 2005. Cytometry 68, 45-52). Here we show selected examples for the utility of two different cytometry-based platforms of epigenetic analysis: ChIP-on-beads, a flow-cytometric test of local histone modifications (Szekvolgyi et al. 2006. Cytometry 69, 1086-1091), and the laser scanning cytometry-based measurement of global epigenetic modifications that might help predict clinical behavior in different pathological conditions. We anticipate that such alternative tools may shortly become indispensable in clinical practice, translating the systematic screening of epigenetic tags from basic research into routine diagnostics of HTP demand.

Original languageEnglish
Title of host publicationChromatin Immunoprecipitation Assays
Subtitle of host publicationMethods and Protocols
EditorsPhilippe Collas
Pages99-111
Number of pages13
DOIs
Publication statusPublished - Dec 1 2009

Publication series

NameMethods in Molecular Biology
Volume567
ISSN (Print)1064-3745

    Fingerprint

Keywords

  • ChIP-on-beads
  • Chromatin immunoprecipitation (ChIP)
  • flow cytometry
  • laser scanning cytometry (LSC)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Szekvolgyi, L., Imre, L., Quang Minh, D. X., Hegedus, E., Bacso, Z., & Szabo, G. (2009). Flow cytometric and laser scanning microscopic approaches in epigenetics research. In P. Collas (Ed.), Chromatin Immunoprecipitation Assays: Methods and Protocols (pp. 99-111). (Methods in Molecular Biology; Vol. 567). https://doi.org/10.1007/978-1-60327-414-2_7