Flow cytometric analysis of proteoglycan expression on murine tumor cells with different metastatic capacity

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Proteoglycan epitopes recognized by poly- or monoclonal antibodies were studied at the cell surface of 3LL murine tumor cell lines with different metastatic capacity. A decreased expression of heparan sulphate and chondroitin sulphate core protein epitopes was observed in the highly metastatic variant HM compared to the low metastatic counter-part LM. Interestingly, the biochemical analysis demonstrated an increased glycosaminoglycan - especially heparan sulphate - production in highly metastatic HM cells. A similar decrease in heparan sulphate proteoglycan core epitope expression was observed in the highly metastatic B16F10 cell line compared to its low metastatic variant F1. The under-representation of proteoglycan core protein epitopes at the cell surface of higly metastatic murine tumor cell lines could be interpreted by the increased glycosylation, or by the loss of those particular epitopes.

Original languageEnglish
Pages (from-to)785-787
Number of pages3
JournalAnticancer Research
Volume10
Issue number3
Publication statusPublished - 1990

Fingerprint

Proteoglycans
Epitopes
Heparitin Sulfate
Neoplasms
Tumor Cell Line
Heparan Sulfate Proteoglycans
Chondroitin Sulfates
Glycosaminoglycans
Glycosylation
Proteins
Monoclonal Antibodies
Cell Line

Keywords

  • Flow cytometry
  • Immunochemistry
  • Metastasis
  • Proteoglycans

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

@article{dbfd2772b06f4c88bbae5bc62a48bac0,
title = "Flow cytometric analysis of proteoglycan expression on murine tumor cells with different metastatic capacity",
abstract = "Proteoglycan epitopes recognized by poly- or monoclonal antibodies were studied at the cell surface of 3LL murine tumor cell lines with different metastatic capacity. A decreased expression of heparan sulphate and chondroitin sulphate core protein epitopes was observed in the highly metastatic variant HM compared to the low metastatic counter-part LM. Interestingly, the biochemical analysis demonstrated an increased glycosaminoglycan - especially heparan sulphate - production in highly metastatic HM cells. A similar decrease in heparan sulphate proteoglycan core epitope expression was observed in the highly metastatic B16F10 cell line compared to its low metastatic variant F1. The under-representation of proteoglycan core protein epitopes at the cell surface of higly metastatic murine tumor cell lines could be interpreted by the increased glycosylation, or by the loss of those particular epitopes.",
keywords = "Flow cytometry, Immunochemistry, Metastasis, Proteoglycans",
author = "J. T{\'i}m{\'a}r and K. Lapis",
year = "1990",
language = "English",
volume = "10",
pages = "785--787",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "3",

}

TY - JOUR

T1 - Flow cytometric analysis of proteoglycan expression on murine tumor cells with different metastatic capacity

AU - Tímár, J.

AU - Lapis, K.

PY - 1990

Y1 - 1990

N2 - Proteoglycan epitopes recognized by poly- or monoclonal antibodies were studied at the cell surface of 3LL murine tumor cell lines with different metastatic capacity. A decreased expression of heparan sulphate and chondroitin sulphate core protein epitopes was observed in the highly metastatic variant HM compared to the low metastatic counter-part LM. Interestingly, the biochemical analysis demonstrated an increased glycosaminoglycan - especially heparan sulphate - production in highly metastatic HM cells. A similar decrease in heparan sulphate proteoglycan core epitope expression was observed in the highly metastatic B16F10 cell line compared to its low metastatic variant F1. The under-representation of proteoglycan core protein epitopes at the cell surface of higly metastatic murine tumor cell lines could be interpreted by the increased glycosylation, or by the loss of those particular epitopes.

AB - Proteoglycan epitopes recognized by poly- or monoclonal antibodies were studied at the cell surface of 3LL murine tumor cell lines with different metastatic capacity. A decreased expression of heparan sulphate and chondroitin sulphate core protein epitopes was observed in the highly metastatic variant HM compared to the low metastatic counter-part LM. Interestingly, the biochemical analysis demonstrated an increased glycosaminoglycan - especially heparan sulphate - production in highly metastatic HM cells. A similar decrease in heparan sulphate proteoglycan core epitope expression was observed in the highly metastatic B16F10 cell line compared to its low metastatic variant F1. The under-representation of proteoglycan core protein epitopes at the cell surface of higly metastatic murine tumor cell lines could be interpreted by the increased glycosylation, or by the loss of those particular epitopes.

KW - Flow cytometry

KW - Immunochemistry

KW - Metastasis

KW - Proteoglycans

UR - http://www.scopus.com/inward/record.url?scp=0025309344&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025309344&partnerID=8YFLogxK

M3 - Article

C2 - 1695080

AN - SCOPUS:0025309344

VL - 10

SP - 785

EP - 787

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 3

ER -