Flow cytometric analysis of cell membrane events induced by interferon-alpha

E. Bálint, A. Veres, I. Ocsovszki, I. Béládi, Z. Várkonyi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Laser-based flow cytometer permits prompt experimental monitoring of fluctuations in the molecular status of cell surface membranes and can "report" the occurrence of early signal transduction events of interferons. The interferons are important regulatory molecules, capable of affecting a number of cell functions in addition to the much studied proliferation of the variety of cell types, inhibition of viral replication and growth. Clinically there is increasing importance of anticancer and antiviral activity of interferon-alpha (IFN). IFN acts primarily by interaction with membrane receptors during signal transduction. The aim of this study was to pursue the importance of the cell membrane changes in the signal transduction of IFN from receptor binding to biological effect. Studies were therefore undertaken to measure the absolute membrane potential of IFN treated cells. The absolute membrane potential of human cells was quantitated by flow cytometry using a voltage-sensitive oxonol dye. The relationship of plasma membrane biophysical properties to the expression of IFN effects was investigated on different types of model cells (U937 monocytes and Daudi lymphoblast). Our studies have suggested changes in the plasma membrane environment after IFN treatment, including changes in the ion flux, membrane potential, alteration of microviscosity, etc.

Original languageEnglish
Pages (from-to)509-512
Number of pages4
JournalLaser Physics
Volume10
Issue number2
Publication statusPublished - Mar 2000

Fingerprint

interferon
Interferons
Cell membranes
membranes
Signal transduction
Membranes
cells
Cells
monocytes
biological effects
cytometry
Flow cytometry
Dyes
dyes
occurrences
Fluxes
Molecules
Lasers
Monitoring

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)
  • Atomic and Molecular Physics, and Optics

Cite this

Flow cytometric analysis of cell membrane events induced by interferon-alpha. / Bálint, E.; Veres, A.; Ocsovszki, I.; Béládi, I.; Várkonyi, Z.

In: Laser Physics, Vol. 10, No. 2, 03.2000, p. 509-512.

Research output: Contribution to journalArticle

Bálint, E. ; Veres, A. ; Ocsovszki, I. ; Béládi, I. ; Várkonyi, Z. / Flow cytometric analysis of cell membrane events induced by interferon-alpha. In: Laser Physics. 2000 ; Vol. 10, No. 2. pp. 509-512.
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