Flavonoids isolated from rumex aquaticus exhibit neuroprotective and neurorestorative properties by enhancing neurite outgrowth and synaptophysin

Orsolya Orbán-Gyapai, Aparna Raghavan, A. Vasas, P. Forgó, J. Hohmann, Zahoor A. Shah

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

There is heightened interest in the field of stroke recovery as there is need for agents that would prevent the debilitating effects of the disorder, thereby tremendously reducing the societal and economic costs associated with it. In this study, the isolation of two flavonoids - quercetin-3-O-galactoside (1) and quercetin-3-O-arabinoside (2) - from Rumex aquaticus (western dock) and their neuroprotective effects were reported in the oxygen-glucose deprivation (OGD) model of in vitro ischemia using rat pheochromocytoma (PC12) cell line. Bioassay-guided fractionation of the ethyl-acetate extract of Rumex aquaticus L. afforded the isolation of compounds 1 and 2. The structures of compounds were established on the basis of spectroscopic analyses (UV, mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR). Both compounds were isolated for the first time from this species. In the course of the pharmacological experiments it was detected that these flavonoids at 10 μM concentration significantly improved cell survival in the oxygen-glucose deprivation model of ischemia. Moreover, they also increased neurite outgrowth in differentiated PC12 cells subjected to ischemic insult. Investigations on the cellular mechanism for the observed effect revealed that compound 1 (10 μM) enhances the expression of synaptophysin - a marker of synapses, and an indicator of synaptic plasticity. Rapid restoration of neurological function following injury is paramount to the prevention of debilitating consequences of ischemic stroke. This combination of neuroprotection and neuritogenic potential could be particularly useful in the recovery phase of stroke.

Original languageEnglish
Pages (from-to)1458-1464
Number of pages7
JournalCNS and Neurological Disorders - Drug Targets
Volume13
Issue number8
Publication statusPublished - Jan 1 2014

Fingerprint

Rumex
Synaptophysin
PC12 Cells
Flavonoids
Stroke
Ischemia
Oxygen
Glucose
Neuronal Plasticity
Quercetin
Neuroprotective Agents
Biological Assay
Synapses
Mass Spectrometry
Cell Survival
Magnetic Resonance Spectroscopy
Economics
Pharmacology
Costs and Cost Analysis
Wounds and Injuries

Keywords

  • Neurite outgrowth
  • Neuroprotection
  • Rumex aquaticus
  • Synaptophysin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pharmacology

Cite this

Flavonoids isolated from rumex aquaticus exhibit neuroprotective and neurorestorative properties by enhancing neurite outgrowth and synaptophysin. / Orbán-Gyapai, Orsolya; Raghavan, Aparna; Vasas, A.; Forgó, P.; Hohmann, J.; Shah, Zahoor A.

In: CNS and Neurological Disorders - Drug Targets, Vol. 13, No. 8, 01.01.2014, p. 1458-1464.

Research output: Contribution to journalArticle

@article{35ef855265b64dcda3ae59e4608b100a,
title = "Flavonoids isolated from rumex aquaticus exhibit neuroprotective and neurorestorative properties by enhancing neurite outgrowth and synaptophysin",
abstract = "There is heightened interest in the field of stroke recovery as there is need for agents that would prevent the debilitating effects of the disorder, thereby tremendously reducing the societal and economic costs associated with it. In this study, the isolation of two flavonoids - quercetin-3-O-galactoside (1) and quercetin-3-O-arabinoside (2) - from Rumex aquaticus (western dock) and their neuroprotective effects were reported in the oxygen-glucose deprivation (OGD) model of in vitro ischemia using rat pheochromocytoma (PC12) cell line. Bioassay-guided fractionation of the ethyl-acetate extract of Rumex aquaticus L. afforded the isolation of compounds 1 and 2. The structures of compounds were established on the basis of spectroscopic analyses (UV, mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR). Both compounds were isolated for the first time from this species. In the course of the pharmacological experiments it was detected that these flavonoids at 10 μM concentration significantly improved cell survival in the oxygen-glucose deprivation model of ischemia. Moreover, they also increased neurite outgrowth in differentiated PC12 cells subjected to ischemic insult. Investigations on the cellular mechanism for the observed effect revealed that compound 1 (10 μM) enhances the expression of synaptophysin - a marker of synapses, and an indicator of synaptic plasticity. Rapid restoration of neurological function following injury is paramount to the prevention of debilitating consequences of ischemic stroke. This combination of neuroprotection and neuritogenic potential could be particularly useful in the recovery phase of stroke.",
keywords = "Neurite outgrowth, Neuroprotection, Rumex aquaticus, Synaptophysin",
author = "Orsolya Orb{\'a}n-Gyapai and Aparna Raghavan and A. Vasas and P. Forg{\'o} and J. Hohmann and Shah, {Zahoor A.}",
year = "2014",
month = "1",
day = "1",
language = "English",
volume = "13",
pages = "1458--1464",
journal = "CNS and Neurological Disorders - Drug Targets",
issn = "1871-5273",
publisher = "Bentham Science Publishers B.V.",
number = "8",

}

TY - JOUR

T1 - Flavonoids isolated from rumex aquaticus exhibit neuroprotective and neurorestorative properties by enhancing neurite outgrowth and synaptophysin

AU - Orbán-Gyapai, Orsolya

AU - Raghavan, Aparna

AU - Vasas, A.

AU - Forgó, P.

AU - Hohmann, J.

AU - Shah, Zahoor A.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - There is heightened interest in the field of stroke recovery as there is need for agents that would prevent the debilitating effects of the disorder, thereby tremendously reducing the societal and economic costs associated with it. In this study, the isolation of two flavonoids - quercetin-3-O-galactoside (1) and quercetin-3-O-arabinoside (2) - from Rumex aquaticus (western dock) and their neuroprotective effects were reported in the oxygen-glucose deprivation (OGD) model of in vitro ischemia using rat pheochromocytoma (PC12) cell line. Bioassay-guided fractionation of the ethyl-acetate extract of Rumex aquaticus L. afforded the isolation of compounds 1 and 2. The structures of compounds were established on the basis of spectroscopic analyses (UV, mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR). Both compounds were isolated for the first time from this species. In the course of the pharmacological experiments it was detected that these flavonoids at 10 μM concentration significantly improved cell survival in the oxygen-glucose deprivation model of ischemia. Moreover, they also increased neurite outgrowth in differentiated PC12 cells subjected to ischemic insult. Investigations on the cellular mechanism for the observed effect revealed that compound 1 (10 μM) enhances the expression of synaptophysin - a marker of synapses, and an indicator of synaptic plasticity. Rapid restoration of neurological function following injury is paramount to the prevention of debilitating consequences of ischemic stroke. This combination of neuroprotection and neuritogenic potential could be particularly useful in the recovery phase of stroke.

AB - There is heightened interest in the field of stroke recovery as there is need for agents that would prevent the debilitating effects of the disorder, thereby tremendously reducing the societal and economic costs associated with it. In this study, the isolation of two flavonoids - quercetin-3-O-galactoside (1) and quercetin-3-O-arabinoside (2) - from Rumex aquaticus (western dock) and their neuroprotective effects were reported in the oxygen-glucose deprivation (OGD) model of in vitro ischemia using rat pheochromocytoma (PC12) cell line. Bioassay-guided fractionation of the ethyl-acetate extract of Rumex aquaticus L. afforded the isolation of compounds 1 and 2. The structures of compounds were established on the basis of spectroscopic analyses (UV, mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR). Both compounds were isolated for the first time from this species. In the course of the pharmacological experiments it was detected that these flavonoids at 10 μM concentration significantly improved cell survival in the oxygen-glucose deprivation model of ischemia. Moreover, they also increased neurite outgrowth in differentiated PC12 cells subjected to ischemic insult. Investigations on the cellular mechanism for the observed effect revealed that compound 1 (10 μM) enhances the expression of synaptophysin - a marker of synapses, and an indicator of synaptic plasticity. Rapid restoration of neurological function following injury is paramount to the prevention of debilitating consequences of ischemic stroke. This combination of neuroprotection and neuritogenic potential could be particularly useful in the recovery phase of stroke.

KW - Neurite outgrowth

KW - Neuroprotection

KW - Rumex aquaticus

KW - Synaptophysin

UR - http://www.scopus.com/inward/record.url?scp=84925728487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925728487&partnerID=8YFLogxK

M3 - Article

C2 - 25345505

AN - SCOPUS:84925728487

VL - 13

SP - 1458

EP - 1464

JO - CNS and Neurological Disorders - Drug Targets

JF - CNS and Neurological Disorders - Drug Targets

SN - 1871-5273

IS - 8

ER -