Flagellin based biomimetic coatings: From cell-repellent surfaces to highly adhesive coatings

Boglarka Kovacs, Daniel Patko, Inna Szekacs, Norbert Orgovan, Sandor Kurunczi, A. Sulyok, Nguyen Quoc Khanh, Balazs Toth, F. Vonderviszt, R. Horváth

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Biomimetic coatings with cell-adhesion-regulating functionalities are intensively researched today. For example, cell-based biosensing for drug development, biomedical implants, and tissue engineering require that the surface adhesion of living cells is well controlled. Recently, we have shown that the bacterial flagellar protein, flagellin, adsorbs through its terminal segments to hydrophobic surfaces, forming an oriented monolayer and exposing its variable D3 domain to the solution. Here, we hypothesized that this nanostructured layer is highly cell-repellent since it mimics the surface of the flagellar filaments. Moreover, we proposed flagellin as a carrier molecule to display the cell-adhesive RGD (Arg-Gly-Asp) peptide sequence and induce cell adhesion on the coated surface. The D3 domain of flagellin was replaced with one or more RGD motifs linked by various oligopeptides modulating flexibility and accessibility of the inserted segment. The obtained flagellin variants were applied to create surface coatings inducing cell adhesion and spreading to different levels, while wild-type flagellin was shown to form a surface layer with strong anti-adhesive properties. As reference surfaces synthetic polymers were applied which have anti-adhesive (PLL-g-PEG poly(L-lysine)-graft-poly(ethylene glycol)) or adhesion inducing properties (RGD-functionalized PLL-g-PEG). Quantitative adhesion data was obtained by employing optical biochips and microscopy. Cell-adhesion-regulating coatings can be simply formed on hydrophobic surfaces by using the developed flagellin-based constructs. The developed novel RGD-displaying flagellin variants can be easily obtained by bacterial production and can serve as alternatives to create cell-adhesion-regulating biomimetic coatings. Statement of Significance In the present work, we show for the first time that - an oriented and dense monolayer of flagellin molecules mimics the surface of the bacterial flagellar filament. Consequently, the fabricated layer is completely cell repellent.- By genetically modifying flagellin, we incorporate cell adhesion regulating functionalities into this anti-adhesive coating.- We can easily tune the adhesion of living cells from completely cell repellent to highly adhesive.

Original languageEnglish
Pages (from-to)66-76
Number of pages11
JournalActa Biomaterialia
Volume42
DOIs
Publication statusPublished - Sep 15 2016

Fingerprint

Flagellin
Biomimetics
Adhesives
Cell Adhesion
Cell adhesion
Coatings
Adhesion
Polyethylene glycols
Phase locked loops
Monolayers
Cells
Biomedical Engineering
Biochips
Oligopeptides
Molecules
Bacterial Proteins
Tissue Engineering
Tissue engineering
Grafts
Peptides

Keywords

  • Biomimetic film
  • Cell adhesion
  • Flagellin
  • Hydrophobic surface modification
  • RGD motif
  • RGD-displaying flagellin
  • Tunable surface coating

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering
  • Molecular Biology

Cite this

Flagellin based biomimetic coatings : From cell-repellent surfaces to highly adhesive coatings. / Kovacs, Boglarka; Patko, Daniel; Szekacs, Inna; Orgovan, Norbert; Kurunczi, Sandor; Sulyok, A.; Khanh, Nguyen Quoc; Toth, Balazs; Vonderviszt, F.; Horváth, R.

In: Acta Biomaterialia, Vol. 42, 15.09.2016, p. 66-76.

Research output: Contribution to journalArticle

Kovacs, Boglarka ; Patko, Daniel ; Szekacs, Inna ; Orgovan, Norbert ; Kurunczi, Sandor ; Sulyok, A. ; Khanh, Nguyen Quoc ; Toth, Balazs ; Vonderviszt, F. ; Horváth, R. / Flagellin based biomimetic coatings : From cell-repellent surfaces to highly adhesive coatings. In: Acta Biomaterialia. 2016 ; Vol. 42. pp. 66-76.
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