FISH chromosome mapping allowing karyotype analysis in Medicago truncatula lines Jemalong J5 and R-108-1

Malika Cerbah, Zoltan Kevei, Sonja Siljak-Yakovlev, É. Kondorosi, Adam Kondorosi, Toan Hanh Trinh

Research output: Contribution to journalArticle

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Abstract

Two lines of Medicago truncatula, R-108-1 and Jemalong J5, exhibit differences in their symbiotic properties, morphological markers, and capacity for regeneration and transformation. Using cytogenetic techniques, we detected differences in chromomycin A3 staining on the two sides of the secondary constriction of the nucleolar chromosome pair of J5 and R-108-1, while DAPI (4', 6-diamidino-2-phenylindole) staining revealed no differences between the two lines. FISH (fluorescent in situ hybridization) analysis showed a difference between J5 and R-108-1 for the same 5S rRNA genes; three loci were found in J5 but only two loci in R-108-1. However, for the 18S-5.8S-26S rDNA genes only a single locus was found in both lines. Based on these analyses, a karyotype for the two lines was established.

Original languageEnglish
Pages (from-to)947-950
Number of pages4
JournalMolecular Plant-Microbe Interactions
Volume12
Issue number11
Publication statusPublished - Nov 1999

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Medicago truncatula
Chromosome Mapping
Chromosomes
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
Karyotype
karyotyping
chromosome mapping
Chromomycin A3
Genes
Staining and Labeling
loci
Cytogenetic Analysis
Ribosomal DNA
rRNA Genes
Constriction
Regeneration
cytogenetics
genes
ribosomal RNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Microbiology

Cite this

FISH chromosome mapping allowing karyotype analysis in Medicago truncatula lines Jemalong J5 and R-108-1. / Cerbah, Malika; Kevei, Zoltan; Siljak-Yakovlev, Sonja; Kondorosi, É.; Kondorosi, Adam; Trinh, Toan Hanh.

In: Molecular Plant-Microbe Interactions, Vol. 12, No. 11, 11.1999, p. 947-950.

Research output: Contribution to journalArticle

Cerbah, M, Kevei, Z, Siljak-Yakovlev, S, Kondorosi, É, Kondorosi, A & Trinh, TH 1999, 'FISH chromosome mapping allowing karyotype analysis in Medicago truncatula lines Jemalong J5 and R-108-1', Molecular Plant-Microbe Interactions, vol. 12, no. 11, pp. 947-950.
Cerbah, Malika ; Kevei, Zoltan ; Siljak-Yakovlev, Sonja ; Kondorosi, É. ; Kondorosi, Adam ; Trinh, Toan Hanh. / FISH chromosome mapping allowing karyotype analysis in Medicago truncatula lines Jemalong J5 and R-108-1. In: Molecular Plant-Microbe Interactions. 1999 ; Vol. 12, No. 11. pp. 947-950.
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AU - Kondorosi, É.

AU - Kondorosi, Adam

AU - Trinh, Toan Hanh

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