First successful vitrification of salmonid ovarian tissue

Jelena Lujić, Zoran Marinović, Simona Sušnik Bajec, Ida Djurdjevič, Eszter Kása, B. Urbányi, A. Horváth

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11 Citations (Scopus)

Abstract

Due to a lack of cryopreservation protocols for fish eggs and embryos, alternative techniques which will enable storage of female genetic resources are crucial for future development of reproduction management in conservation biology and aquaculture. Experiments were conducted to develop an optimal vitrification protocol for cryopreservation of brown trout Salmo trutta juvenile ovarian tissue. Needle immersed vitrification (NIV) method was used where ovaries were pinned on an acupuncture needle, passaged through equilibration and vitrification solutions containing different combinations and concentrations of methanol (MeOH), propylene glycol (PG) and dimethyl sulfoxide (Me2SO) and subsequently plunged into liquid nitrogen. Vitrification solutions containing equal cryoprotectant concentrations (3M Me2SO and 3M PG) yielded the highest oogonia survival rates (up to 40%) and qualitatively and quantitatively unaltered perinucleolar follicles. The method developed for brown trout could be applied to the conservation of female genetic resources of other salmonid species, including endangered and endemic species or populations.

Original languageEnglish
Pages (from-to)154-157
Number of pages4
JournalCryobiology
Volume76
DOIs
Publication statusPublished - Jun 1 2017

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Keywords

  • Brown trout
  • Cryopreservation
  • Needle immersed vitrification
  • Oogonia
  • Salmo trutta

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Lujić, J., Marinović, Z., Sušnik Bajec, S., Djurdjevič, I., Kása, E., Urbányi, B., & Horváth, A. (2017). First successful vitrification of salmonid ovarian tissue. Cryobiology, 76, 154-157. https://doi.org/10.1016/j.cryobiol.2017.04.005