First report of Cryptosporidium deer-like genotype in Malaysian cattle

N. Abdul Halim, J. Plutzer, M. A. Bakheit, P. Karanis

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Fifty faecal samples from diarrheic calves between 1 and 6 months old were collected per rectum from 5 farms around Petaling District in Selangor, Malaysia for Cryptosporidium species detection and genotyping investigation. Oocysts were purified using sedimentation and gradient centrifugation, then examined by immunofluorescence assay (IFAT). Genomic DNA was extracted from all samples and nested PCR was performed to amplify the SSU rRNA gene. Eighteen samples (36%) were positive for Cryptosporidium species by PCR. The sequence and phylogenetic analysis of 14 isolates indicated that Cryptosporidium parvum was most common (11 isolates) followed by Cryptosporidium deer-like genotype (3 isolates). The present work reports the first data on Cryptosporidium genotyping from cattle in Malaysia.

Original languageEnglish
Pages (from-to)325-329
Number of pages5
JournalVeterinary Parasitology
Volume152
Issue number3-4
DOIs
Publication statusPublished - Apr 15 2008

Fingerprint

Cryptosporidium
Deer
deer
Genotype
genotype
cattle
Malaysia
genotyping
Cryptosporidium parvum
Polymerase Chain Reaction
Oocysts
rectum
Centrifugation
rRNA Genes
oocysts
Rectum
sampling
centrifugation
fluorescent antibody technique
Fluorescent Antibody Technique

Keywords

  • Cryptosporidium spp. cattle
  • Genotype
  • IFAT
  • Malaysia

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Parasitology
  • veterinary(all)

Cite this

First report of Cryptosporidium deer-like genotype in Malaysian cattle. / Halim, N. Abdul; Plutzer, J.; Bakheit, M. A.; Karanis, P.

In: Veterinary Parasitology, Vol. 152, No. 3-4, 15.04.2008, p. 325-329.

Research output: Contribution to journalArticle

Halim, N. Abdul ; Plutzer, J. ; Bakheit, M. A. ; Karanis, P. / First report of Cryptosporidium deer-like genotype in Malaysian cattle. In: Veterinary Parasitology. 2008 ; Vol. 152, No. 3-4. pp. 325-329.
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