First report and genetic characterization of porcine astroviruses of lineage 4 and 2 in diarrhoeic pigs in India

Jobin Jose Kattoor, Yashpal Singh Malik, Sharad Saurabh, Shubhankar Sircar, Obli Rajendran Vinodhkumar, Durlav Prasad Bora, Kuldeep Dhama, Souvik Ghosh, Krisztian Banyai, Nadia Touil, Ahmed S Abdel-Moneim, Anastasia N. Vlasova, Nobumichi Kobayashi, Raj Kumar Singh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Porcine astroviruses (PAstVs) have extended their distribution globally and have a high prevalence; however, their clinical significance is still under investigation. Thus far, information about their prevalence and diversity in the Indian pig population is unknown. This study is the first report on the prevalence and genetic characterization of PAstVs in diarrhoeic piglets in India. From January 2013 to December 2017, 757 samples were screened using an RT-PCR assay and PAstV infection was detected in 17.6% (133/757) pigs. Of the 133 positive samples, 79 (59.4%) were positive for PAstV alone, whereas 54 (40.6%) were found to be co-infected with porcine rotavirus A (PoRVA). Phylogenetic analysis of RdRp/capsid gene region revealed high genetic heterogeneity among PAstV sequences, with a predominance of PAstV lineage 4 and detection of lineage 2. The lineage 4 PAstVs exhibited 61.2%–94.5% sequence similarity at the nucleotide level to other reported sequences, whereas lineage 2 strain shared 66.0%–71.6% sequence identity with cognate sequences of the same lineage. This is the first report on PAstV and circulation of lineages 4 and 2 in India. Further, phylogenetic analysis indicates a multiphyletic origin of PAstV strains and suggests cross-border circulation of PAstVs with a similar genetic configuration in Asian countries.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalTransboundary and Emerging Diseases
Volume66
Issue number1
DOIs
Publication statusPublished - Jan 2019

Keywords

  • India
  • Porcine astroviruses
  • co-infection
  • epidemiology
  • genetic diversity
  • lineages
  • virus detection

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • veterinary(all)

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  • Cite this

    Kattoor, J. J., Malik, Y. S., Saurabh, S., Sircar, S., Vinodhkumar, O. R., Bora, D. P., Dhama, K., Ghosh, S., Banyai, K., Touil, N., Abdel-Moneim, AS., Vlasova, A. N., Kobayashi, N., & Singh, R. K. (2019). First report and genetic characterization of porcine astroviruses of lineage 4 and 2 in diarrhoeic pigs in India. Transboundary and Emerging Diseases, 66(1), 47-53. https://doi.org/10.1111/tbed.13058