First molecular identification of Mycoplasma ovis and 'Candidatus M. haemoovis' from goat, with lack of haemoplasma PCR-positivity in lice

S. Hornok, István Hajtós, Marina L. Meli, Imre Farkas, Eniko Gönczi, Theres Meili, Regina Hofmann-Lehmann

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In order to investigate haemotropic Mycoplasma (formerly Eperythrozoon) infection of goats, blood samples and blood-sucking lice (Linognathus stenopsis) were collected in two goat herds. DNA was extracted from 20 blood samples and from 49 lice allocated to six pools according to host individuals. Haemoplasma infection was detected in four goats by real-time PCR. From the sample with the highest bacterial load the simultaneous presence of M. ovis and 'Candidatus M. haemoovis' was demonstrated by cloning and sequencing. Louse pools were haemoplasma negative, including those from bacteraemic animals. However, not only were Anaplasma inclusion bodies seen in blood smears from goats, but relevant PCR-positivity was also detected among lice. This is the first report of a molecular investigation on caprine haemoplasmas, including analysis of their bloodsucking lice. In summary, goats are susceptible to both molecularly characterised ovine haemoplasmas. On the other hand, goat sucking lice (L. stenopsis) do not appear to be potential vectors of these agents.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalActa Veterinaria Hungarica
Volume60
Issue number3
DOIs
Publication statusPublished - Sep 1 2012

Fingerprint

Linognathus stenopsis
Anoplura
Mycoplasma ovis
Mycoplasma
Goats
Sheep
goats
Polymerase Chain Reaction
Anaplasma
blood
Phthiraptera
Bacterial Load
hematophagy
Inclusion Bodies
Infection
lice
inclusion bodies
Organism Cloning
Real-Time Polymerase Chain Reaction
sampling

Keywords

  • eperythrozoon
  • goat
  • haemoplasma
  • Mycoplasma

ASJC Scopus subject areas

  • veterinary(all)

Cite this

First molecular identification of Mycoplasma ovis and 'Candidatus M. haemoovis' from goat, with lack of haemoplasma PCR-positivity in lice. / Hornok, S.; Hajtós, István; Meli, Marina L.; Farkas, Imre; Gönczi, Eniko; Meili, Theres; Hofmann-Lehmann, Regina.

In: Acta Veterinaria Hungarica, Vol. 60, No. 3, 01.09.2012, p. 355-360.

Research output: Contribution to journalArticle

Hornok, S. ; Hajtós, István ; Meli, Marina L. ; Farkas, Imre ; Gönczi, Eniko ; Meili, Theres ; Hofmann-Lehmann, Regina. / First molecular identification of Mycoplasma ovis and 'Candidatus M. haemoovis' from goat, with lack of haemoplasma PCR-positivity in lice. In: Acta Veterinaria Hungarica. 2012 ; Vol. 60, No. 3. pp. 355-360.
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