A lovak fertozo arteritisét okozó vírus elso hazai izolálása

Translated title of the contribution: First isolation of equine infectious arteritis virus in Hungary

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The authors demonstrated the presence of equine infectious arteritis virus in Hungary years ago with PCR-method but isolation has not been reported yet in this country, because the causative agent is relatively difficult to isolate. In the previous one and a half year semen samples from seropositive stallions (191), organ samples and foetal membranes from aborted foetuses (25) and nasal discharge and white blood cell samples (3) were examined. Using virus isolation with semi-liquid culture medium, they managed to isolate virus in 20 cases from 219 samples. There was no significant difference in relation of positivity in the different sample groups: they isolated arteritis virus in 18 cases from semen samples (9.42%) and in 2 cases from organ samples (8%), while they did not manage to isolate virus from nasal discharge and white blood cell samples. According to their observation, without using semi-liquid culture medium CPE of virus is more difficult or not to be observed. Other important finding that ultrasonic treatment of semen samples helps very much virus isolation: from 18 certainly positive samples after ultrasonic treatment all of them were found positive, while without ultrasonic treatment only 2 of them were found positive. Parallelly to virus isolation experiments they developped RT-PCR-method with the same effectiveness as isolation. According to the authors, the state of chronic virus-spreading horses can be established certainly only by using the two methods together. On the basis of the results of virus isolation and parallel PCR-method it can be stated that the proportion of virus carrying of seropositive stallions in Hungary is lower than it was found in the eighties and nineties in North-America.

Original languageHungarian
Pages (from-to)729-734
Number of pages6
JournalMagyar Allatorvosok Lapja
Volume123
Issue number12
Publication statusPublished - 2001

Fingerprint

Equine Arteritis Virus
arteritis
Hungary
Viruses
horses
viruses
ultrasonic treatment
Semen
sampling
Ultrasonics
semen
Nose
stallions
Polymerase Chain Reaction
Culture Media
Leukocytes
leukocytes
culture media
Aborted Fetus
Extraembryonic Membranes

ASJC Scopus subject areas

  • veterinary(all)

Cite this

A lovak fertozo arteritisét okozó vírus elso hazai izolálása. / Hornyák, A.; Bakonyi, T.; Pálfi, V.; Rusvai, M.

In: Magyar Allatorvosok Lapja, Vol. 123, No. 12, 2001, p. 729-734.

Research output: Contribution to journalArticle

@article{c7ef1531bcd042619b77b449fc12e918,
title = "A lovak fertozo arteritis{\'e}t okoz{\'o} v{\'i}rus elso hazai izol{\'a}l{\'a}sa",
abstract = "The authors demonstrated the presence of equine infectious arteritis virus in Hungary years ago with PCR-method but isolation has not been reported yet in this country, because the causative agent is relatively difficult to isolate. In the previous one and a half year semen samples from seropositive stallions (191), organ samples and foetal membranes from aborted foetuses (25) and nasal discharge and white blood cell samples (3) were examined. Using virus isolation with semi-liquid culture medium, they managed to isolate virus in 20 cases from 219 samples. There was no significant difference in relation of positivity in the different sample groups: they isolated arteritis virus in 18 cases from semen samples (9.42{\%}) and in 2 cases from organ samples (8{\%}), while they did not manage to isolate virus from nasal discharge and white blood cell samples. According to their observation, without using semi-liquid culture medium CPE of virus is more difficult or not to be observed. Other important finding that ultrasonic treatment of semen samples helps very much virus isolation: from 18 certainly positive samples after ultrasonic treatment all of them were found positive, while without ultrasonic treatment only 2 of them were found positive. Parallelly to virus isolation experiments they developped RT-PCR-method with the same effectiveness as isolation. According to the authors, the state of chronic virus-spreading horses can be established certainly only by using the two methods together. On the basis of the results of virus isolation and parallel PCR-method it can be stated that the proportion of virus carrying of seropositive stallions in Hungary is lower than it was found in the eighties and nineties in North-America.",
author = "A. Horny{\'a}k and T. Bakonyi and V. P{\'a}lfi and M. Rusvai",
year = "2001",
language = "Hungarian",
volume = "123",
pages = "729--734",
journal = "Magyar Allatorvosok Lapja",
issn = "0025-004X",
publisher = "Magyar Mezogazdasag Ltd",
number = "12",

}

TY - JOUR

T1 - A lovak fertozo arteritisét okozó vírus elso hazai izolálása

AU - Hornyák, A.

AU - Bakonyi, T.

AU - Pálfi, V.

AU - Rusvai, M.

PY - 2001

Y1 - 2001

N2 - The authors demonstrated the presence of equine infectious arteritis virus in Hungary years ago with PCR-method but isolation has not been reported yet in this country, because the causative agent is relatively difficult to isolate. In the previous one and a half year semen samples from seropositive stallions (191), organ samples and foetal membranes from aborted foetuses (25) and nasal discharge and white blood cell samples (3) were examined. Using virus isolation with semi-liquid culture medium, they managed to isolate virus in 20 cases from 219 samples. There was no significant difference in relation of positivity in the different sample groups: they isolated arteritis virus in 18 cases from semen samples (9.42%) and in 2 cases from organ samples (8%), while they did not manage to isolate virus from nasal discharge and white blood cell samples. According to their observation, without using semi-liquid culture medium CPE of virus is more difficult or not to be observed. Other important finding that ultrasonic treatment of semen samples helps very much virus isolation: from 18 certainly positive samples after ultrasonic treatment all of them were found positive, while without ultrasonic treatment only 2 of them were found positive. Parallelly to virus isolation experiments they developped RT-PCR-method with the same effectiveness as isolation. According to the authors, the state of chronic virus-spreading horses can be established certainly only by using the two methods together. On the basis of the results of virus isolation and parallel PCR-method it can be stated that the proportion of virus carrying of seropositive stallions in Hungary is lower than it was found in the eighties and nineties in North-America.

AB - The authors demonstrated the presence of equine infectious arteritis virus in Hungary years ago with PCR-method but isolation has not been reported yet in this country, because the causative agent is relatively difficult to isolate. In the previous one and a half year semen samples from seropositive stallions (191), organ samples and foetal membranes from aborted foetuses (25) and nasal discharge and white blood cell samples (3) were examined. Using virus isolation with semi-liquid culture medium, they managed to isolate virus in 20 cases from 219 samples. There was no significant difference in relation of positivity in the different sample groups: they isolated arteritis virus in 18 cases from semen samples (9.42%) and in 2 cases from organ samples (8%), while they did not manage to isolate virus from nasal discharge and white blood cell samples. According to their observation, without using semi-liquid culture medium CPE of virus is more difficult or not to be observed. Other important finding that ultrasonic treatment of semen samples helps very much virus isolation: from 18 certainly positive samples after ultrasonic treatment all of them were found positive, while without ultrasonic treatment only 2 of them were found positive. Parallelly to virus isolation experiments they developped RT-PCR-method with the same effectiveness as isolation. According to the authors, the state of chronic virus-spreading horses can be established certainly only by using the two methods together. On the basis of the results of virus isolation and parallel PCR-method it can be stated that the proportion of virus carrying of seropositive stallions in Hungary is lower than it was found in the eighties and nineties in North-America.

UR - http://www.scopus.com/inward/record.url?scp=0347081900&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0347081900&partnerID=8YFLogxK

M3 - Article

VL - 123

SP - 729

EP - 734

JO - Magyar Allatorvosok Lapja

JF - Magyar Allatorvosok Lapja

SN - 0025-004X

IS - 12

ER -