First genetic characterization of Usutu virus from Culex pipiens mosquitoes Serbia, 2014

Gábor Kemenesi, Dóra Buzás, Brigitta Zana, Kornélia Kurucz, Bosiljka Krtinic, Anett Kepner, Fanni Földes, F. Jakab

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Since its first appearance in Europe, Usutu virus (USUV) diverged to several different genetic lineages. The virus was reported to date from multiple countries across Europe (Hungary, Italy, Switzerland, Spain, Germany, Czech Republic and Belgium). Considering the more frequently published impact of the virus on humans it is crucial to investigate locally circulating genetic variants and trace its evolution. We retrospectively analyzed mosquito samples from Serbia Vojvodina region, collected during 2014. In this study we report the results of the screening of 23,753 female mosquitoes (753 pools) for USUV-specific nucleic-acid. Out of the 753 pools sampled, the presence of USUV RNA was confirmed in 3 pools of Culex pipiens mosquitoes, collected in August. Based on their partial NS5 sequence, all strains were identical, therefore we adjusted one representative strain for complete genome sequencing. Based on phylogenetic analysis the Serbian USUV sequences were most closely related to the virus that emerged in Austria in 2001, in Hungary in 2005 and was circulating until 2015 in Hungary. This data presents a wider geographic distribution of this genetic variant and provides the first genetic data from this region.

Original languageEnglish
Pages (from-to)58-61
Number of pages4
JournalInfection, Genetics and Evolution
Volume63
DOIs
Publication statusPublished - Sep 1 2018

Fingerprint

Usutu virus
Serbia
Culex
Culex pipiens
Culicidae
mosquito
virus
Hungary
Viruses
viruses
Belgium
Austria
Czech Republic
nucleic acids
Switzerland
Yugoslavia
geographical distribution
Germany
RNA Viruses
Italy

Keywords

  • Culicidae
  • European lineage 1
  • Mosquito monitoring
  • Vector

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

First genetic characterization of Usutu virus from Culex pipiens mosquitoes Serbia, 2014. / Kemenesi, Gábor; Buzás, Dóra; Zana, Brigitta; Kurucz, Kornélia; Krtinic, Bosiljka; Kepner, Anett; Földes, Fanni; Jakab, F.

In: Infection, Genetics and Evolution, Vol. 63, 01.09.2018, p. 58-61.

Research output: Contribution to journalArticle

Kemenesi, Gábor ; Buzás, Dóra ; Zana, Brigitta ; Kurucz, Kornélia ; Krtinic, Bosiljka ; Kepner, Anett ; Földes, Fanni ; Jakab, F. / First genetic characterization of Usutu virus from Culex pipiens mosquitoes Serbia, 2014. In: Infection, Genetics and Evolution. 2018 ; Vol. 63. pp. 58-61.
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