First comprehensive phylogenetic analysis of the genus Erysiphe (Erysiphales, Erysiphaceae) I. The Microsphaera lineage

Susumu Takamatsu, Hanako Ito, Yoshiaki Shiroya, L. Kiss, Vasyl Heluta

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The genus Erysiphe (including powdery mildew fungi only known as anamorph, Pseudoidium) is the largest genus in the Erysiphaceae and contains more than 50% of all species in this family. Little is known about the phylogenetic structure of this genus. We conducted a comprehensive phylogenetic analysis of the Microsphaera-lineage, a monophyletic group including species of sects. Microsphaera and Erysiphe, using 401 sequences of nuc ITS1-5.8S-ITS2 and the 28S rDNA regions. This analysis gave many small clades delimited by the host plant genus or family. We identified two deep branches, albeit with moderate bootstrap supports, that divided the 401 sequences into three large groups. In addition, we identified four large clades consisting of homogeneous sequences of powdery mildews from a wide range of host plants beyond family level, namely, the E. aquilegiae clade, the E. alphitoides clade, the E. quercicola clade, and the E. trifoliorum s. lat. clade. Isolates from herbaceous plants were mostly situated in the E. aquilegiae clade and in Group III that was located at the most derived position of the Microsphaeralineage. On the other hand, the basal part of the Microsphaera-lineage was occupied by isolates from woody plants except for E. glycines that was used as an outgroup taxon. This supports our previous hypothesis that tree-parasitic powdery mildews are phylogenetically primitive in the Erysiphaceae in general, and host-shift from trees to herbs occurred many times independently during the evolution of powdery mildews. Molecular clock analyses suggested that the divergence of the Microsphaera-lineage began ca. 20 million years ago in the Miocene Epoch of the Neogene Period.

Original languageEnglish
Pages (from-to)475-489
Number of pages15
JournalMycologia
Volume107
Issue number3
DOIs
Publication statusPublished - 2015

Fingerprint

Erysiphaceae
Erysiphales
mildews
phylogenetics
herb
host plant
phylogeny
host plants
woody plant
Neogene
anamorphs
herbaceous plants
Ribosomal DNA
divergence
powdery mildew
woody plants
Miocene
fungus
Glycine
herbs

Keywords

  • 28S rDNA
  • Host relationship
  • ITS
  • Powdery mildew
  • Pseudoidium

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Physiology
  • Medicine(all)

Cite this

First comprehensive phylogenetic analysis of the genus Erysiphe (Erysiphales, Erysiphaceae) I. The Microsphaera lineage. / Takamatsu, Susumu; Ito, Hanako; Shiroya, Yoshiaki; Kiss, L.; Heluta, Vasyl.

In: Mycologia, Vol. 107, No. 3, 2015, p. 475-489.

Research output: Contribution to journalArticle

Takamatsu, Susumu ; Ito, Hanako ; Shiroya, Yoshiaki ; Kiss, L. ; Heluta, Vasyl. / First comprehensive phylogenetic analysis of the genus Erysiphe (Erysiphales, Erysiphaceae) I. The Microsphaera lineage. In: Mycologia. 2015 ; Vol. 107, No. 3. pp. 475-489.
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