First cloned human immortalized adipose derived mesenchymal stem-cell line with chimeric SS18-SSX1 gene (SS-iASC)

Dóra Mihály, Zsolt Matula, Yi Che Changchien, Gergő Papp, P. Tátrai, Z. Sápi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The SS18-SSX chimeric gene is unique to synovial sarcoma. Multiple model systems including mouse cell lines expressing SS18-SSX, and genetically engineered mouse models of synovial sarcoma have been developed to elucidate the role of the chimeric gene in synovial sarcomagenesis. Although several cell lines stably expressing human SS18-SSX exist, there is an ongoing need for cell culture models enabling researchers to investigate the molecular mechanism of SS18-SSX action in a relevant cellular context. Here we report the establishment of a novel SS18-SSX1-expressing cell line created from immortalized human adipose tissue-derived mesenchymal stem cells via lentiviral transduction of the chimeric gene. Our cell line, termed SS-iASC, has been characterized by karyotyping and cell line identification, and stable expression of SS18-SSX1 has been verified using real-time PCR (RT-PCR), nested PCR, immunofluorescence, and immunoblotting. Focal cytokeratin positivity characteristic of synovial sarcoma but no β-Catenin, Bcl-2 or cyclin D1 expression was observed in SS-iASC. The novel cell line expressing SS18-SSX1 on a human adipose-derived stromal cell background is expected to be helpful in addressing the question whether the chimeric gene alone is sufficient to trigger the formation of synovial sarcoma.

Original languageEnglish
Pages (from-to)52-60
Number of pages9
JournalCancer genetics
Volume216-217
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Mesenchymal Stromal Cells
Synovial Sarcoma
Cell Line
Genes
Catenins
Karyotyping
Cyclin D1
Stromal Cells
Keratins
Immunoblotting
Fluorescent Antibody Technique
Adipose Tissue
Real-Time Polymerase Chain Reaction
Cell Culture Techniques
Research Personnel
Polymerase Chain Reaction

Keywords

  • adipose-derived mesenchymal stem cells
  • SS18-SSX chimeric gene
  • SS18-SSX1-expressing human cell line
  • synovial sarcoma

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

First cloned human immortalized adipose derived mesenchymal stem-cell line with chimeric SS18-SSX1 gene (SS-iASC). / Mihály, Dóra; Matula, Zsolt; Changchien, Yi Che; Papp, Gergő; Tátrai, P.; Sápi, Z.

In: Cancer genetics, Vol. 216-217, 01.10.2017, p. 52-60.

Research output: Contribution to journalArticle

Mihály, Dóra ; Matula, Zsolt ; Changchien, Yi Che ; Papp, Gergő ; Tátrai, P. ; Sápi, Z. / First cloned human immortalized adipose derived mesenchymal stem-cell line with chimeric SS18-SSX1 gene (SS-iASC). In: Cancer genetics. 2017 ; Vol. 216-217. pp. 52-60.
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