Abstract
The SS18-SSX chimeric gene is unique to synovial sarcoma. Multiple model systems including mouse cell lines expressing SS18-SSX, and genetically engineered mouse models of synovial sarcoma have been developed to elucidate the role of the chimeric gene in synovial sarcomagenesis. Although several cell lines stably expressing human SS18-SSX exist, there is an ongoing need for cell culture models enabling researchers to investigate the molecular mechanism of SS18-SSX action in a relevant cellular context. Here we report the establishment of a novel SS18-SSX1-expressing cell line created from immortalized human adipose tissue-derived mesenchymal stem cells via lentiviral transduction of the chimeric gene. Our cell line, termed SS-iASC, has been characterized by karyotyping and cell line identification, and stable expression of SS18-SSX1 has been verified using real-time PCR (RT-PCR), nested PCR, immunofluorescence, and immunoblotting. Focal cytokeratin positivity characteristic of synovial sarcoma but no β-Catenin, Bcl-2 or cyclin D1 expression was observed in SS-iASC. The novel cell line expressing SS18-SSX1 on a human adipose-derived stromal cell background is expected to be helpful in addressing the question whether the chimeric gene alone is sufficient to trigger the formation of synovial sarcoma.
| Original language | English |
|---|---|
| Pages (from-to) | 52-60 |
| Number of pages | 9 |
| Journal | Cancer genetics |
| Volume | 216-217 |
| DOIs | |
| Publication status | Published - Oct 1 2017 |
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Keywords
- adipose-derived mesenchymal stem cells
- SS18-SSX chimeric gene
- SS18-SSX1-expressing human cell line
- synovial sarcoma
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cancer Research
Cite this
First cloned human immortalized adipose derived mesenchymal stem-cell line with chimeric SS18-SSX1 gene (SS-iASC). / Mihály, Dóra; Matula, Zsolt; Changchien, Yi Che; Papp, Gergő; Tátrai, P.; Sápi, Z.
In: Cancer genetics, Vol. 216-217, 01.10.2017, p. 52-60.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - First cloned human immortalized adipose derived mesenchymal stem-cell line with chimeric SS18-SSX1 gene (SS-iASC)
AU - Mihály, Dóra
AU - Matula, Zsolt
AU - Changchien, Yi Che
AU - Papp, Gergő
AU - Tátrai, P.
AU - Sápi, Z.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - The SS18-SSX chimeric gene is unique to synovial sarcoma. Multiple model systems including mouse cell lines expressing SS18-SSX, and genetically engineered mouse models of synovial sarcoma have been developed to elucidate the role of the chimeric gene in synovial sarcomagenesis. Although several cell lines stably expressing human SS18-SSX exist, there is an ongoing need for cell culture models enabling researchers to investigate the molecular mechanism of SS18-SSX action in a relevant cellular context. Here we report the establishment of a novel SS18-SSX1-expressing cell line created from immortalized human adipose tissue-derived mesenchymal stem cells via lentiviral transduction of the chimeric gene. Our cell line, termed SS-iASC, has been characterized by karyotyping and cell line identification, and stable expression of SS18-SSX1 has been verified using real-time PCR (RT-PCR), nested PCR, immunofluorescence, and immunoblotting. Focal cytokeratin positivity characteristic of synovial sarcoma but no β-Catenin, Bcl-2 or cyclin D1 expression was observed in SS-iASC. The novel cell line expressing SS18-SSX1 on a human adipose-derived stromal cell background is expected to be helpful in addressing the question whether the chimeric gene alone is sufficient to trigger the formation of synovial sarcoma.
AB - The SS18-SSX chimeric gene is unique to synovial sarcoma. Multiple model systems including mouse cell lines expressing SS18-SSX, and genetically engineered mouse models of synovial sarcoma have been developed to elucidate the role of the chimeric gene in synovial sarcomagenesis. Although several cell lines stably expressing human SS18-SSX exist, there is an ongoing need for cell culture models enabling researchers to investigate the molecular mechanism of SS18-SSX action in a relevant cellular context. Here we report the establishment of a novel SS18-SSX1-expressing cell line created from immortalized human adipose tissue-derived mesenchymal stem cells via lentiviral transduction of the chimeric gene. Our cell line, termed SS-iASC, has been characterized by karyotyping and cell line identification, and stable expression of SS18-SSX1 has been verified using real-time PCR (RT-PCR), nested PCR, immunofluorescence, and immunoblotting. Focal cytokeratin positivity characteristic of synovial sarcoma but no β-Catenin, Bcl-2 or cyclin D1 expression was observed in SS-iASC. The novel cell line expressing SS18-SSX1 on a human adipose-derived stromal cell background is expected to be helpful in addressing the question whether the chimeric gene alone is sufficient to trigger the formation of synovial sarcoma.
KW - adipose-derived mesenchymal stem cells
KW - SS18-SSX chimeric gene
KW - SS18-SSX1-expressing human cell line
KW - synovial sarcoma
UR - http://www.scopus.com/inward/record.url?scp=85026799282&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85026799282&partnerID=8YFLogxK
U2 - 10.1016/j.cancergen.2017.07.003
DO - 10.1016/j.cancergen.2017.07.003
M3 - Article
VL - 216-217
SP - 52
EP - 60
JO - Cancer genetics
JF - Cancer genetics
SN - 2210-7762
ER -