First characterisation of flavonoid- and diarylheptanoid-type antioxidant phenolics in Corylus maxima by HPLC-DAD-ESI-MS

Eszter Riethmüller, Gergo Tóth, Ágnes Alberti, Krisztina Végh, Ilaria Burlini, Árpád Könczöl, György Tibor Balogh, A. Kéry

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Corylus maxima Mill. (Betulaceae) leaves have been used in traditional medicine both internally and externally, nevertheless phytochemical exploration of the plant remains incomplete. In this study, the in vitro antioxidant activity and polyphenolic composition of the ethyl acetate and methanolic extracts of C. maxima leaves and bark are reported for the first time. The radical scavenging activities of the extracts were investigated by the ABTS and DPPH assays. All the extracts of C. maxima possessed notable antioxidant activity. By mean of a HPLC-DAD-ESI-TOF and a HPLC-DAD-ESI-MS/MS method, altogether twenty-two phenolics were tentatively characterised: one flavan derivative (. 1), seven flavonol derivatives (. 4, 6, 12, 13, 16, 20 and 21) and fourteen diarylheptanoids (. 2, 3, 5, 7-. 11, 14, 15, 17-. 19 and 22). The amount of the two main flavonoids - myricetin-3-. O-rhamnoside (. 6) and quercetin-3-. O-rhamnoside (. 13) - and two diarylheptanoids - oregonin (. 3) and hirsutenone (. 15) - in the extracts were determined by a validated HPLC-ESI-MS/MS method in multiple reaction monitoring (MRM) mode. Our results showed that C. maxima could be considered as a valuable source of pharmacologically important natural products that might contribute to the revaluation of the phytotherapeutical potential of the plant.

Original languageEnglish
Pages (from-to)159-167
Number of pages9
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume107
DOIs
Publication statusPublished - Mar 5 2015

Fingerprint

Diarylheptanoids
Corylus
Flavonoids
Antioxidants
High Pressure Liquid Chromatography
Derivatives
Scavenging
Quercetin
Phytochemicals
Betulaceae
Biological Products
Medicine
Assays
Traditional Medicine
Monitoring
Chemical analysis

Keywords

  • Corylus maxima
  • Diarylheptanoid
  • Flavonoid
  • HPLC
  • MS

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry
  • Medicine(all)

Cite this

First characterisation of flavonoid- and diarylheptanoid-type antioxidant phenolics in Corylus maxima by HPLC-DAD-ESI-MS. / Riethmüller, Eszter; Tóth, Gergo; Alberti, Ágnes; Végh, Krisztina; Burlini, Ilaria; Könczöl, Árpád; Balogh, György Tibor; Kéry, A.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 107, 05.03.2015, p. 159-167.

Research output: Contribution to journalArticle

Riethmüller, Eszter ; Tóth, Gergo ; Alberti, Ágnes ; Végh, Krisztina ; Burlini, Ilaria ; Könczöl, Árpád ; Balogh, György Tibor ; Kéry, A. / First characterisation of flavonoid- and diarylheptanoid-type antioxidant phenolics in Corylus maxima by HPLC-DAD-ESI-MS. In: Journal of Pharmaceutical and Biomedical Analysis. 2015 ; Vol. 107. pp. 159-167.
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AU - Alberti, Ágnes

AU - Végh, Krisztina

AU - Burlini, Ilaria

AU - Könczöl, Árpád

AU - Balogh, György Tibor

AU - Kéry, A.

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