Fine-tuning of the flowering time control in winter barley: The importance of HvOS2 and HvVRN2 in non-inductive conditions

Arantxa Monteagudo, Ernesto Igartua, Bruno Contreras-Moreira, M. Pilar Gracia, Javier Ramos, I. Karsai, Ana M. Casas

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: In winter barley plants, vernalization and photoperiod cues have to be integrated to promote flowering. Plant development and expression of different flowering promoter (HvVRN1, HvCO2, PPD-H1, HvFT1, HvFT3) and repressor (HvVRN2, HvCO9 and HvOS2) genes were evaluated in two winter barley varieties under: (1) natural increasing photoperiod, without vernalization, and (2) under short day conditions in three insufficient vernalization treatments. These challenging conditions were chosen to capture non-optimal and natural responses, representative of those experienced in the Mediterranean area. Results: In absence of vernalization and under increasing photoperiods, HvVRN2 expression increased with day-length, mainly between 12 and 13 h photoperiods in our latitudes. The flowering promoter gene in short days, HvFT3, was only expressed after receiving induction of cold or plant age, which was associated with low transcript levels of HvVRN2 and HvOS2. Under the sub-optimal conditions here described, great differences in development were found between the two winter barley varieties used in the study. Delayed development in 'Barberousse' was associated with increased expression levels of HvOS2. Novel variation for HvCO9 and HvOS2 is reported and might explain such differences. Conclusions: The balance between the expression of flowering promoters and repressor genes regulates the promotion towards flowering or the maintenance of the vegetative state. HvOS2, an ortholog of FLC, appears as a strong candidate to mediate in the vernalization response of barley. Natural variation found would help to exploit the plasticity in development to obtain better-adapted varieties for current and future climate conditions.

Original languageEnglish
Article number113
JournalBMC Plant Biology
Volume19
Issue number1
DOIs
Publication statusPublished - Mar 25 2019

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winter barley
vernalization
photoperiod
flowering
promoter regions
genes
plant age
plant development
barley
climate

Keywords

  • Barley
  • Gene expression
  • HvCO2
  • HvFT3
  • HvOS2
  • HvVRN1
  • HvVRN2
  • Photoperiod
  • PPD-H1
  • Vernalization

ASJC Scopus subject areas

  • Plant Science

Cite this

Fine-tuning of the flowering time control in winter barley : The importance of HvOS2 and HvVRN2 in non-inductive conditions. / Monteagudo, Arantxa; Igartua, Ernesto; Contreras-Moreira, Bruno; Gracia, M. Pilar; Ramos, Javier; Karsai, I.; Casas, Ana M.

In: BMC Plant Biology, Vol. 19, No. 1, 113, 25.03.2019.

Research output: Contribution to journalArticle

Monteagudo, Arantxa ; Igartua, Ernesto ; Contreras-Moreira, Bruno ; Gracia, M. Pilar ; Ramos, Javier ; Karsai, I. ; Casas, Ana M. / Fine-tuning of the flowering time control in winter barley : The importance of HvOS2 and HvVRN2 in non-inductive conditions. In: BMC Plant Biology. 2019 ; Vol. 19, No. 1.
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abstract = "Background: In winter barley plants, vernalization and photoperiod cues have to be integrated to promote flowering. Plant development and expression of different flowering promoter (HvVRN1, HvCO2, PPD-H1, HvFT1, HvFT3) and repressor (HvVRN2, HvCO9 and HvOS2) genes were evaluated in two winter barley varieties under: (1) natural increasing photoperiod, without vernalization, and (2) under short day conditions in three insufficient vernalization treatments. These challenging conditions were chosen to capture non-optimal and natural responses, representative of those experienced in the Mediterranean area. Results: In absence of vernalization and under increasing photoperiods, HvVRN2 expression increased with day-length, mainly between 12 and 13 h photoperiods in our latitudes. The flowering promoter gene in short days, HvFT3, was only expressed after receiving induction of cold or plant age, which was associated with low transcript levels of HvVRN2 and HvOS2. Under the sub-optimal conditions here described, great differences in development were found between the two winter barley varieties used in the study. Delayed development in 'Barberousse' was associated with increased expression levels of HvOS2. Novel variation for HvCO9 and HvOS2 is reported and might explain such differences. Conclusions: The balance between the expression of flowering promoters and repressor genes regulates the promotion towards flowering or the maintenance of the vegetative state. HvOS2, an ortholog of FLC, appears as a strong candidate to mediate in the vernalization response of barley. Natural variation found would help to exploit the plasticity in development to obtain better-adapted varieties for current and future climate conditions.",
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AU - Monteagudo, Arantxa

AU - Igartua, Ernesto

AU - Contreras-Moreira, Bruno

AU - Gracia, M. Pilar

AU - Ramos, Javier

AU - Karsai, I.

AU - Casas, Ana M.

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AB - Background: In winter barley plants, vernalization and photoperiod cues have to be integrated to promote flowering. Plant development and expression of different flowering promoter (HvVRN1, HvCO2, PPD-H1, HvFT1, HvFT3) and repressor (HvVRN2, HvCO9 and HvOS2) genes were evaluated in two winter barley varieties under: (1) natural increasing photoperiod, without vernalization, and (2) under short day conditions in three insufficient vernalization treatments. These challenging conditions were chosen to capture non-optimal and natural responses, representative of those experienced in the Mediterranean area. Results: In absence of vernalization and under increasing photoperiods, HvVRN2 expression increased with day-length, mainly between 12 and 13 h photoperiods in our latitudes. The flowering promoter gene in short days, HvFT3, was only expressed after receiving induction of cold or plant age, which was associated with low transcript levels of HvVRN2 and HvOS2. Under the sub-optimal conditions here described, great differences in development were found between the two winter barley varieties used in the study. Delayed development in 'Barberousse' was associated with increased expression levels of HvOS2. Novel variation for HvCO9 and HvOS2 is reported and might explain such differences. Conclusions: The balance between the expression of flowering promoters and repressor genes regulates the promotion towards flowering or the maintenance of the vegetative state. HvOS2, an ortholog of FLC, appears as a strong candidate to mediate in the vernalization response of barley. Natural variation found would help to exploit the plasticity in development to obtain better-adapted varieties for current and future climate conditions.

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KW - HvOS2

KW - HvVRN1

KW - HvVRN2

KW - Photoperiod

KW - PPD-H1

KW - Vernalization

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