Fine-tuning of helper T cell activation and apoptosis by antigen-presenting cells

Katalin Ludanyi, P. Gogolák, Bence Rethi, M. Magócsi, Cynthia Detre, J. Matkó, E. Rajnavolgyi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The role of antigen-presenting cells (APC) in regulating helper T cell responses and activation-induced cell death (AICD) was investigated in vitro. T cell activation was monitored by measuring the early rise of intracellular free calcium [Ca+]ic, mRNA and cell surface expression of activation and apoptotic molecules, the production of cytokines and the activation of transcription factors. Our results demonstrate that the unique characteristics of a given APC can modify the threshold, kinetics and magnitude of the T cell response. The rapid and sustained rise of intracellular free calcium correlated well with the extent of cytokine production and the expression of activation molecules. Fas-dependent AICD could be induced by the most potent antigen-presenting cell (2PK3) only. Our results demonstrate that the response and fate of effector/memory CD4+ helper T lymphocytes is highly dependent on the individual properties of the APC they encounter.

Original languageEnglish
Pages (from-to)939-950
Number of pages12
JournalCellular Signalling
Volume16
Issue number8
DOIs
Publication statusPublished - Aug 2004

Fingerprint

CD27 Antigens
Antigen-Presenting Cells
Helper-Inducer T-Lymphocytes
Apoptosis
Cell Death
Cytokines
Calcium
T-Lymphocytes
Transcription Factors
Messenger RNA

Keywords

  • AICD
  • Antigen presentation
  • Antigen-specific activation
  • APC
  • Co-stimulation
  • Helper T cell
  • T cell signaling
  • TCR

ASJC Scopus subject areas

  • Cell Biology

Cite this

Fine-tuning of helper T cell activation and apoptosis by antigen-presenting cells. / Ludanyi, Katalin; Gogolák, P.; Rethi, Bence; Magócsi, M.; Detre, Cynthia; Matkó, J.; Rajnavolgyi, E.

In: Cellular Signalling, Vol. 16, No. 8, 08.2004, p. 939-950.

Research output: Contribution to journalArticle

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AU - Rajnavolgyi, E.

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