Fine structure and synaptology of the nitrergic neurons in medial septum of the rat brain

K. Halasy, Balázs Szoke, Gergely Janzsó

Research output: Contribution to journalArticle

Abstract

The nitrergic neuron population and certain aspects of their connectivity (peptidergic inputs, co-localization with GABA, synaptic target distribution) were studied in the medial septum of the rat brain. The histochemical localization of NADPH diaphorase and immunohistochemical identification of nNOS at light and electron microscopic level was applied. Double-labeling experiments with galanin and leucine enkephalin, moreover the postembedding GABA immunogold staining was also carried out. NADPH diaphorase-and nNOS-immunopositive neurons could be identified inside the borders of medial septum. Out of their peptidergic inputs galanin-and leucine enkephaline-immunopositive varicose fibers were found in close apposition with nNOS-immunopositive neurons. Based on fine structural characteristics (large indented nucleus, thin cytoplasmic rim, lack of axosomatic synapses) the nitrergic neurons are suggested to be identical with the septal cholinergic nerve cells. Their boutons established asymmetrical synapses mainly on dendritic shafts and spines, some of which were also nNOS-immunopositive. A lower amount of nNOS-immunopositive boutons of presumably extrinsic origin were found to be GABAergic.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalActa Biologica Hungarica
Volume68
Issue number1
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

Nitrergic Neurons
Septum of Brain
shaft
Neurons
Galanin
NADPH Dehydrogenase
connectivity
brain
Rats
Brain
electron
Synapses
gamma-Aminobutyric Acid
Leucine Enkephalin
Dendritic Spines
experiment
Leucine
Cholinergic Agents
Electrons
Staining and Labeling

Keywords

  • GABA-synaptology
  • NADPH
  • Neuropeptides
  • NNOS

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Fine structure and synaptology of the nitrergic neurons in medial septum of the rat brain. / Halasy, K.; Szoke, Balázs; Janzsó, Gergely.

In: Acta Biologica Hungarica, Vol. 68, No. 1, 01.03.2017, p. 1-13.

Research output: Contribution to journalArticle

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