Fine structural localization of calcium binding sites in the neuromuscular junction

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Abstract

By means of electron cytochemical analysis of rat neuromuscular junctions subjected to perfusion with a calcium-containing (5 mM) aldehyde fixative, two kinds of presynaptic calcium binding sites were demonstrated. 'A' sites are located above openings of junctional folds; these triangular elements are identical with presynaptic protrusions of the active zone and probably comprise calcium channels of the presynaptic membrane. 'B' sites, located within the terminal axoplasm, are associated with multivesicular bodies probably accumulating surplus intracellular calcium. This fine structural arrangement enables a highly parsimonious usage of extracellular calcium in inducing release of acetylcholine from synaptic vesicles. Junctional folds of the postsynaptic membrane may funnel calcium ions exactly to spatially restricted calcium channels. Having entered the terminal through the channels opened by the arrival of nerve action potential, calcium ions may exert their action upon synaptic vesicles accumulated in the closest vicinity by means of stripping off hydration barriers and by inducing membrane fusions, that finally result in a quantal release of acetylcholine from the terminal.

Original languageEnglish
Pages (from-to)49-56
Number of pages8
JournalActa Biologica Academiae Scientiarum Hungaricae
Volume31
Issue number1-3
Publication statusPublished - 1980

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Neuromuscular Junction
binding sites
calcium
Binding Sites
Calcium
calcium channels
Synaptic Vesicles
acetylcholine
Calcium Channels
Membranes
Acetylcholine
membrane
vesicle
Ions
ions
Multivesicular Bodies
Fixatives
Membrane Fusion
action potentials
fold

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

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abstract = "By means of electron cytochemical analysis of rat neuromuscular junctions subjected to perfusion with a calcium-containing (5 mM) aldehyde fixative, two kinds of presynaptic calcium binding sites were demonstrated. 'A' sites are located above openings of junctional folds; these triangular elements are identical with presynaptic protrusions of the active zone and probably comprise calcium channels of the presynaptic membrane. 'B' sites, located within the terminal axoplasm, are associated with multivesicular bodies probably accumulating surplus intracellular calcium. This fine structural arrangement enables a highly parsimonious usage of extracellular calcium in inducing release of acetylcholine from synaptic vesicles. Junctional folds of the postsynaptic membrane may funnel calcium ions exactly to spatially restricted calcium channels. Having entered the terminal through the channels opened by the arrival of nerve action potential, calcium ions may exert their action upon synaptic vesicles accumulated in the closest vicinity by means of stripping off hydration barriers and by inducing membrane fusions, that finally result in a quantal release of acetylcholine from the terminal.",
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AU - Csillik, B.

AU - Knyihár-Csillik, E.

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N2 - By means of electron cytochemical analysis of rat neuromuscular junctions subjected to perfusion with a calcium-containing (5 mM) aldehyde fixative, two kinds of presynaptic calcium binding sites were demonstrated. 'A' sites are located above openings of junctional folds; these triangular elements are identical with presynaptic protrusions of the active zone and probably comprise calcium channels of the presynaptic membrane. 'B' sites, located within the terminal axoplasm, are associated with multivesicular bodies probably accumulating surplus intracellular calcium. This fine structural arrangement enables a highly parsimonious usage of extracellular calcium in inducing release of acetylcholine from synaptic vesicles. Junctional folds of the postsynaptic membrane may funnel calcium ions exactly to spatially restricted calcium channels. Having entered the terminal through the channels opened by the arrival of nerve action potential, calcium ions may exert their action upon synaptic vesicles accumulated in the closest vicinity by means of stripping off hydration barriers and by inducing membrane fusions, that finally result in a quantal release of acetylcholine from the terminal.

AB - By means of electron cytochemical analysis of rat neuromuscular junctions subjected to perfusion with a calcium-containing (5 mM) aldehyde fixative, two kinds of presynaptic calcium binding sites were demonstrated. 'A' sites are located above openings of junctional folds; these triangular elements are identical with presynaptic protrusions of the active zone and probably comprise calcium channels of the presynaptic membrane. 'B' sites, located within the terminal axoplasm, are associated with multivesicular bodies probably accumulating surplus intracellular calcium. This fine structural arrangement enables a highly parsimonious usage of extracellular calcium in inducing release of acetylcholine from synaptic vesicles. Junctional folds of the postsynaptic membrane may funnel calcium ions exactly to spatially restricted calcium channels. Having entered the terminal through the channels opened by the arrival of nerve action potential, calcium ions may exert their action upon synaptic vesicles accumulated in the closest vicinity by means of stripping off hydration barriers and by inducing membrane fusions, that finally result in a quantal release of acetylcholine from the terminal.

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