Fine specificity of a rabbit antibody interacting with human IgG Fc receptor-like molecules

Z. Rozsnyay, G. Sármay, I. Szabó, Gy Medgyesi, G. Gorini, J. Gergely

Research output: Contribution to journalArticle

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Abstract

A polyclonal rabbit antibody raised against an Fc receptor (FcR)-like membrane glycoprotein fraction of chronic leukaemic lymphocytes has previously been prepared and partially characterized [1]. This antibody, called AbA, was found to precipitate a 70-kDa and a 45-kDa fraction of the detergent lysate of U937 cells and to inhibit ligand binding to FcγR on the P388D1 murine macrophage cell line. In the present work we have characterised this antibody further. All FcγRII-positive B lymphoblastoid cell lines, as well as resting human B lymphocytes, were positively stained with the AbA antibody. U937 cells were found to be negative, but after stimulation with phorbol ester (PMA), 50% of the cells became positive. AbA antibody did not react with human T cell lines or with the T+0 cell subset of peripheral blood. Monocytes were also negative. On the other hand, AbA antibody exhibited a dose-dependent inhibition of antibody-mediated cytotoxic reaction (ADCC) of monocytes, while not affecting K cell-mediated ADCC. It had an inhibitory effect of EA rosette formation of B cells and stimulated U937 cells. Furthermore, it interacted with the soluble form of FcγRII released by activated B lymphocytes, and - similarily to IgG - precipitated a 33 kDa fraction from the supernatant of B cells.

Original languageEnglish
Pages (from-to)303-311
Number of pages9
JournalImmunology Letters
Volume25
Issue number4
DOIs
Publication statusPublished - 1990

Fingerprint

IgG Receptors
Fc Receptors
Rabbits
Antibodies
U937 Cells
B-Lymphocytes
Cell Line
Monocytes
Rosette Formation
Membrane Glycoproteins
T-Lymphocyte Subsets
Phorbol Esters
Detergents
Immunoglobulin G
Macrophages
Lymphocytes
Ligands
T-Lymphocytes

Keywords

  • FcγRII, Human B cell
  • Polyclonal antibody

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Fine specificity of a rabbit antibody interacting with human IgG Fc receptor-like molecules. / Rozsnyay, Z.; Sármay, G.; Szabó, I.; Medgyesi, Gy; Gorini, G.; Gergely, J.

In: Immunology Letters, Vol. 25, No. 4, 1990, p. 303-311.

Research output: Contribution to journalArticle

Rozsnyay, Z. ; Sármay, G. ; Szabó, I. ; Medgyesi, Gy ; Gorini, G. ; Gergely, J. / Fine specificity of a rabbit antibody interacting with human IgG Fc receptor-like molecules. In: Immunology Letters. 1990 ; Vol. 25, No. 4. pp. 303-311.
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AU - Gergely, J.

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AB - A polyclonal rabbit antibody raised against an Fc receptor (FcR)-like membrane glycoprotein fraction of chronic leukaemic lymphocytes has previously been prepared and partially characterized [1]. This antibody, called AbA, was found to precipitate a 70-kDa and a 45-kDa fraction of the detergent lysate of U937 cells and to inhibit ligand binding to FcγR on the P388D1 murine macrophage cell line. In the present work we have characterised this antibody further. All FcγRII-positive B lymphoblastoid cell lines, as well as resting human B lymphocytes, were positively stained with the AbA antibody. U937 cells were found to be negative, but after stimulation with phorbol ester (PMA), 50% of the cells became positive. AbA antibody did not react with human T cell lines or with the T+0 cell subset of peripheral blood. Monocytes were also negative. On the other hand, AbA antibody exhibited a dose-dependent inhibition of antibody-mediated cytotoxic reaction (ADCC) of monocytes, while not affecting K cell-mediated ADCC. It had an inhibitory effect of EA rosette formation of B cells and stimulated U937 cells. Furthermore, it interacted with the soluble form of FcγRII released by activated B lymphocytes, and - similarily to IgG - precipitated a 33 kDa fraction from the supernatant of B cells.

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