Fine-scale mapping of cortical laminar activity during sleep slow oscillations using high-density linear silicon probes

Richárd Fiáth, Bogdan Cristian Raducanu, Silke Musa, Alexandru Andrei, Carolina Mora Lopez, Marleen Welkenhuysen, Patrick Ruther, Arno Aarts, I. Ulbert

Research output: Contribution to journalArticle

Abstract

Background: The cortical slow (∼1 Hz) oscillation (SO), which is thought to play an active role in the consolidation of memories, is a brain rhythm characteristic of slow-wave sleep, with alternating periods of neuronal activity and silence. Although the laminar distribution of cortical activity during SO is well-studied by using linear neural probes, traditional devices have a relatively low (20–100 μm) spatial resolution along cortical layers. New method: In this work, we demonstrate a high-density linear silicon probe fabricated to record the SO with very high spatial resolution (∼6 μm), simultaneously from multiple cortical layers. Ketamine/xylazine-induced SO was acquired acutely from the neocortex of rats, followed by the examination of the high-resolution laminar structure of cortical activity. Results: The probe provided high-quality extracellular recordings, and the obtained cortical laminar profiles of the SO were in good agreement with the literature data. Furthermore, we could record the simultaneous activity of 30–50 cortical single units. Spiking activity of these neurons showed layer-specific differences. Comparison with existing methods: The developed silicon probe measures neuronal activity with at least a three-fold higher spatial resolution compared with traditional linear probes. By exploiting this feature, we could determine the site of up-state initiation with a higher precision than before. Additionally, increased spatial resolution may provide more reliable spike sorting results, as well as a higher single unit yield. Conclusions: The high spatial resolution provided by the electrodes allows to examine the fine structure of local population activity during sleep SO in greater detail.

Original languageEnglish
JournalJournal of Neuroscience Methods
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Silicon
Sleep
Xylazine
Neocortex
Ketamine
Electrodes
Neurons
Equipment and Supplies
Brain
Population
Memory Consolidation

Keywords

  • High-density silicon probe
  • Laminar recording
  • Neocortex
  • Single-unit activity
  • Slow oscillation
  • Slow-wave activity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Fine-scale mapping of cortical laminar activity during sleep slow oscillations using high-density linear silicon probes. / Fiáth, Richárd; Raducanu, Bogdan Cristian; Musa, Silke; Andrei, Alexandru; Lopez, Carolina Mora; Welkenhuysen, Marleen; Ruther, Patrick; Aarts, Arno; Ulbert, I.

In: Journal of Neuroscience Methods, 01.01.2018.

Research output: Contribution to journalArticle

Fiáth, Richárd ; Raducanu, Bogdan Cristian ; Musa, Silke ; Andrei, Alexandru ; Lopez, Carolina Mora ; Welkenhuysen, Marleen ; Ruther, Patrick ; Aarts, Arno ; Ulbert, I. / Fine-scale mapping of cortical laminar activity during sleep slow oscillations using high-density linear silicon probes. In: Journal of Neuroscience Methods. 2018.
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AU - Raducanu, Bogdan Cristian

AU - Musa, Silke

AU - Andrei, Alexandru

AU - Lopez, Carolina Mora

AU - Welkenhuysen, Marleen

AU - Ruther, Patrick

AU - Aarts, Arno

AU - Ulbert, I.

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AB - Background: The cortical slow (∼1 Hz) oscillation (SO), which is thought to play an active role in the consolidation of memories, is a brain rhythm characteristic of slow-wave sleep, with alternating periods of neuronal activity and silence. Although the laminar distribution of cortical activity during SO is well-studied by using linear neural probes, traditional devices have a relatively low (20–100 μm) spatial resolution along cortical layers. New method: In this work, we demonstrate a high-density linear silicon probe fabricated to record the SO with very high spatial resolution (∼6 μm), simultaneously from multiple cortical layers. Ketamine/xylazine-induced SO was acquired acutely from the neocortex of rats, followed by the examination of the high-resolution laminar structure of cortical activity. Results: The probe provided high-quality extracellular recordings, and the obtained cortical laminar profiles of the SO were in good agreement with the literature data. Furthermore, we could record the simultaneous activity of 30–50 cortical single units. Spiking activity of these neurons showed layer-specific differences. Comparison with existing methods: The developed silicon probe measures neuronal activity with at least a three-fold higher spatial resolution compared with traditional linear probes. By exploiting this feature, we could determine the site of up-state initiation with a higher precision than before. Additionally, increased spatial resolution may provide more reliable spike sorting results, as well as a higher single unit yield. Conclusions: The high spatial resolution provided by the electrodes allows to examine the fine structure of local population activity during sleep SO in greater detail.

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