Finding an alternative column for the separation of antibiotics on XTerra RP using a column classification system

Erik Haghedooren, Kristóf Kóczián, Shan Huang, Sanja Dragovic, B. Noszál, Jos Hoogmartens, Erwin Adams

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A project to develop a test procedure to characterize reversed-phase liquid chromatographic C18 columns was started earlier and resulted in a fast, simple, repeatable, and reproducible test procedure. In this paper, the separations of two antibiotics (erythromycin and tetracycline) from their respective impurities were examined. Both methods were developed on XTerra RP, a hybrid column. The performance of the column classification system was evaluated by finding an alternative column for the XTerra RP column. The column classification system was helpful in the selection of a suitable column for the separation of erythromycin, but also showed its limitations towards the separation of tetracycline. The addition of an efficiency parameter and/or omission of one of the original parameters did not improve the results.

Original languageEnglish
Pages (from-to)1081-1103
Number of pages23
JournalJournal of Liquid Chromatography and Related Technologies
Volume31
Issue number8
DOIs
Publication statusPublished - Jan 2008

Fingerprint

Erythromycin
Anti-Bacterial Agents
Tetracycline
Impurities
Liquids

Keywords

  • Erythromycin
  • Reversed-phase liquid chromatographic C columns
  • Test procedure
  • Tetracycline
  • XTerra RP

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Finding an alternative column for the separation of antibiotics on XTerra RP using a column classification system. / Haghedooren, Erik; Kóczián, Kristóf; Huang, Shan; Dragovic, Sanja; Noszál, B.; Hoogmartens, Jos; Adams, Erwin.

In: Journal of Liquid Chromatography and Related Technologies, Vol. 31, No. 8, 01.2008, p. 1081-1103.

Research output: Contribution to journalArticle

Haghedooren, Erik ; Kóczián, Kristóf ; Huang, Shan ; Dragovic, Sanja ; Noszál, B. ; Hoogmartens, Jos ; Adams, Erwin. / Finding an alternative column for the separation of antibiotics on XTerra RP using a column classification system. In: Journal of Liquid Chromatography and Related Technologies. 2008 ; Vol. 31, No. 8. pp. 1081-1103.
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