Fidelity of aminoacylation by rat-liver tyrosyl-tRNA synthetase. Effect of age

R. Takahashi, S. Goto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The possibility of change in the rate of misrecognition of amino acids by rat liver tyrosyl-tRNA synthetase during aging was investigated. Frequency of misrecognition of phenylalanine vs tyrosine was determined at two temperatures by competitive assay using partially purified enzymes. At 25°C, the error frequencies were 5.17 x 10-8 and 8.24 x 10-8 in young and old animals, respectively. These values are much below the reported error frequencies for the prokaryotic enzymes: i.e. ~ 5 x 10-6. Although the fidelity of tyrosyl-tRNA synthetase from old animals appeared to be slightly lower, the difference was not statistically significant. At 37°C, the error frequencies were increased 1.3-1.5-fold, but again the difference between young and old animals was not significant. To our knowledge, this is the first report in which fidelity of aminoacyl-tRNA synthetase of animals of various ages has been compared using natural amino acids.

Original languageEnglish
Pages (from-to)381-386
Number of pages6
JournalEuropean Journal of Biochemistry
Volume178
Issue number2
Publication statusPublished - 1988

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Tyrosine-tRNA Ligase
Aminoacylation
Liver
Rats
Animals
Amino Acyl-tRNA Synthetases
Amino Acids
Enzymes
Phenylalanine
Tyrosine
Assays
Aging of materials
Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

Fidelity of aminoacylation by rat-liver tyrosyl-tRNA synthetase. Effect of age. / Takahashi, R.; Goto, S.

In: European Journal of Biochemistry, Vol. 178, No. 2, 1988, p. 381-386.

Research output: Contribution to journalArticle

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