Fibulin-1 suppression of fibronectin-regulated cell adhesion and motility

Waleed O. Twal, A. Czirók, B. Hegedűs, Christian Knaak, Mastan R. Chintalapudi, Hiroto Okagawa, Yukiko Sugi, W. Scott Argraves

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Fibulin-1 is an extracellular matrix protein often associated with fibronectin (FN) in vivo. In this study, the ability of fibulin-1 to modulate adhesion, spreading and motility-promoting activities of FN was investigated. Fibulin-1 was found to have pronounced inhibitory effects on the cell attachment and spreading promoted by FN. Fibulin-1 was also found to inhibit the motility of a variety of cell types on FN substrata. For example, the FN-dependent haptotactic motility of breast carcinoma (MDA MB231) cells, epidermal carcinoma (A431), melanoma (A375 SM), rat pulmonary aortic smooth muscle cells (PAC1) and Chinese hamster ovary (CHO) cells was inhibited by the presence of fibulin-1 bound to FN-coated Boyden chamber membranes. Cells transfected to overproduce fibulin-1 displayed reduced velocity, distance of movement and persistence time on FN substrata. Similarly, the incorporation of fibulin-1 into FN-containing type I collagen gels inhibited the invasion of endocardial cushion mesenchymal cells migrating from cultured embryonic heart explants. By contrast, incorporation of fibulin-1 into collagen gels lacking FN had no effect on the migration of endocardial cushion cells. These results suggest that the motility-suppressive effects of fibulin-1 might be FN specific. Furthermore, such effects are cell-type specific, in that the migration of gingival fibroblasts and endothelial cells on FN substrata is not responsive to fibulin-1. Additional studies found that the mechanism for the motility-suppressive effects of fibulin-1 does not involve perturbations of interactions between α5β1 or α4 integrins, or heparan sulfate proteoglycans with FN. However, fibulin-1 was found to inhibit extracellular signal regulated kinase (ERK) activation and to suppress phosphorylation of myosin heavy chain. This ability to influence signal transduction cascades that modulate the actin-myosin motor complex might be the basis for the effects of fibulin-1 on adhesion and motility.

Original languageEnglish
Pages (from-to)4587-4598
Number of pages12
JournalJournal of Cell Science
Volume114
Issue number24
Publication statusPublished - 2001

Fingerprint

Fibronectins
Cell Adhesion
Cell Movement
Endocardial Cushions
fibulin
Gels
Heparan Sulfate Proteoglycans
Myosin Heavy Chains
Extracellular Matrix Proteins
Extracellular Signal-Regulated MAP Kinases
Myosins
Collagen Type I
Cricetulus
Integrins
Smooth Muscle Myocytes
Actins
Ovary
Cultured Cells
Melanoma
Signal Transduction

Keywords

  • Actinomyosin
  • Adhesion
  • Fibronectin
  • Fibulin
  • Migration

ASJC Scopus subject areas

  • Cell Biology

Cite this

Twal, W. O., Czirók, A., Hegedűs, B., Knaak, C., Chintalapudi, M. R., Okagawa, H., ... Scott Argraves, W. (2001). Fibulin-1 suppression of fibronectin-regulated cell adhesion and motility. Journal of Cell Science, 114(24), 4587-4598.

Fibulin-1 suppression of fibronectin-regulated cell adhesion and motility. / Twal, Waleed O.; Czirók, A.; Hegedűs, B.; Knaak, Christian; Chintalapudi, Mastan R.; Okagawa, Hiroto; Sugi, Yukiko; Scott Argraves, W.

In: Journal of Cell Science, Vol. 114, No. 24, 2001, p. 4587-4598.

Research output: Contribution to journalArticle

Twal, WO, Czirók, A, Hegedűs, B, Knaak, C, Chintalapudi, MR, Okagawa, H, Sugi, Y & Scott Argraves, W 2001, 'Fibulin-1 suppression of fibronectin-regulated cell adhesion and motility', Journal of Cell Science, vol. 114, no. 24, pp. 4587-4598.
Twal, Waleed O. ; Czirók, A. ; Hegedűs, B. ; Knaak, Christian ; Chintalapudi, Mastan R. ; Okagawa, Hiroto ; Sugi, Yukiko ; Scott Argraves, W. / Fibulin-1 suppression of fibronectin-regulated cell adhesion and motility. In: Journal of Cell Science. 2001 ; Vol. 114, No. 24. pp. 4587-4598.
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