Fibroblasts and lymphocytes from Alzheimer patients are resistant to β-amyloid-induced increase in the intracellular calcium concentration

A. Palotás, J. Kálmán, M. Palotás, Anna Juhász, Z. Janka, Botond Penke

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Abstract

A major neuropathological finding in Alzheimer's disease (AD) is the presence of senile plaques in certain regions in the brain. The plaques contain extracellular deposits of β-amyloid peptide (βAP). Destabilization of intracellular calcium homeostasis in neurons, caused by βAP, plays a central role in AD pathogenesis. In the present study, the authors report ionic alterations of lymphocytes and fibroblasts harvested from sporadic AD patients and from age-matched controls. Intracellular free calcium level ([Ca2+]i) of human cells, labeled with Fura-2AM, was determined by dual wavelength spectrofluorimetry. Basal [Ca2+]i appeared to be higher in AD lymphocytes when compared to control ones. Resting [Ca2+]i of AD fibroblasts, however, has proven to be lower than that seen with control cells. Exposure of cells to βAP resulted in the elevation of the [Ca2+]i in both control cell types, however, that of AD lymphocytes and fibroblasts did not differ considerably.

Original languageEnglish
Pages (from-to)971-974
Number of pages4
JournalProgress in Neuro-Psychopharmacology and Biological Psychiatry
Volume26
Issue number5
DOIs
Publication statusPublished - Jun 2002

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Keywords

  • Alzheimer's disease
  • Fibroblast
  • Fluorescence
  • Lymphocyte
  • β-Amyloid

ASJC Scopus subject areas

  • Pharmacology
  • Biological Psychiatry

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