Fibrin pathways for precursor cell immigration from bone marrow into the osteotomy gap

Konrad Wolf, J. Hamar, S. Moravec, T. Farkas, E. Höcherl, C. Pfister

Research output: Contribution to journalArticle

Abstract

The primary goal of scientific investigations into fracture healing is to achieve a complete structural and functional restoration with research into control mechanisms and early biomechanical influences on the orientation of precursor cell immigration pathways. Primary fibrin networks can exhibit isotropy or anisotropy as a result of retraction triggered by messenger substances and energy carriers. This suggests the existence of a primary biomechanical component that is used in the immigration of precursor cells. The morphometry of fibrin structures gives a mean value of 1.12 ± 0.007 (mean ± SEM), which differs markedly from the hypothetical value of zero, thus demonstrating anisotropy or alignment of the fibrin structures. The morphometric test of precursor cell counts to analyze the dynamics of precursor cell immigration, carried out in the bone marrow between the 10th and 25th days, reveals significant differences between the cell count in the marrow and the osteotomy gap. The greatest movement of mononuclear cells in the marrow takes place on the 15th day, with 4.9 ± 0.2 cells per counting field. In the osteotomy gap, the levels found on the 15 th day were 3.1 ± 0.1, and remained the same until the 25 th day, whereupon a figure of 2.9 ± 0.1 was returned. We interpret the alignment of the fibrin network as an early morphological correlate of a biomechanical component. The administration of platelet-rich plasma (PRP) in human patients seems sensible, and the industry already offers PRP methods for use on human patients (Curasan AG, Kleinostheim, Germany). Thrombocytic factors can be obtained which boost the retraction process and pre-form network structures for precursor cell immigration. Nondifferentiated mononuclear cells migrate along both fine and thick fibrin structures. Growth factors, gene therapy and cell therapy may in future have a positive influence on the complex process of bone healing. Tissue engineering also offers other ways of restoring the original structure of bones in a short space of time.

Original languageEnglish
Pages (from-to)24-35
Number of pages12
JournalApplied Cardiopulmonary Pathophysiology
Volume11
Issue number1
Publication statusPublished - 2007

Fingerprint

Emigration and Immigration
Osteotomy
Fibrin
Bone Marrow Cells
Platelet-Rich Plasma
Bone Marrow
Anisotropy
Cell Count
Bone and Bones
Fracture Healing
Tissue Engineering
Cell- and Tissue-Based Therapy
Genetic Therapy
Cell Movement
Germany
Intercellular Signaling Peptides and Proteins
Industry
Research

Keywords

  • Fibrin pathways
  • Osteoneogenesis
  • Platelet-rich plasma
  • Precursor cell immigration
  • Retraction

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Cardiology and Cardiovascular Medicine
  • Pulmonary and Respiratory Medicine

Cite this

Fibrin pathways for precursor cell immigration from bone marrow into the osteotomy gap. / Wolf, Konrad; Hamar, J.; Moravec, S.; Farkas, T.; Höcherl, E.; Pfister, C.

In: Applied Cardiopulmonary Pathophysiology, Vol. 11, No. 1, 2007, p. 24-35.

Research output: Contribution to journalArticle

Wolf, K, Hamar, J, Moravec, S, Farkas, T, Höcherl, E & Pfister, C 2007, 'Fibrin pathways for precursor cell immigration from bone marrow into the osteotomy gap', Applied Cardiopulmonary Pathophysiology, vol. 11, no. 1, pp. 24-35.
Wolf, Konrad ; Hamar, J. ; Moravec, S. ; Farkas, T. ; Höcherl, E. ; Pfister, C. / Fibrin pathways for precursor cell immigration from bone marrow into the osteotomy gap. In: Applied Cardiopulmonary Pathophysiology. 2007 ; Vol. 11, No. 1. pp. 24-35.
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