αElastin methylester forms with proteoglycan subunits and structural glycoproteins water-insoluble complexes which after staining with phosphotungstic acid (pH 7.0), reveal at large magnification bundles of slender smooth fibrils with a diameter from 4 to 5 nm. Similar fibrils can be observed after interaction of αelastin methylester with chondroitin sulfate, but only if fixation with glutaraldehyde is used prior to staining. Phosphotungstic acid alters the organization of the complexes, in particular those formed with ligands of low molecular weight. The possible biological significance of the interactions studied is discussed.
ASJC Scopus subject areas
- Orthopedics and Sports Medicine
- Molecular Biology
- Cell Biology