Fibre-optic biosensor for hypoxanthine and xanthine based on a chemiluminescence reaction

J. Hlavay, S. D. Haemmerli, G. G. Guilbault

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Fibre-optic biosensors were constructed for determination of hypoxanthine and xanthine. Xanthine oxidase and peroxidase were immobilized on different preactivated membranes which were subsequently mounted onto the tip of a fibre-optic bundle. The H2O2 generated by the reaction of hypoxanthine and xanthine oxidase was measured by chemiluminescence (CL) detection using luminol and peroxidase. A linear calibration curve of the sensors in the range of 1-316 μM hypoxanthine and 3ṡ1-216 μM xanthine, respectively, with a detection limit of 0ṡ55 μM hypoxanthine was obtained. Recovery of hypoxanthine ranged between 91 and 102%.

Original languageEnglish
Pages (from-to)189-195
Number of pages7
JournalBiosensors and Bioelectronics
Volume9
Issue number3
DOIs
Publication statusPublished - 1994

Fingerprint

Hypoxanthine
Xanthine
Chemiluminescence
Biosensing Techniques
Luminescence
Biosensors
Fiber optics
Xanthine Oxidase
Peroxidase
Calibration
Membranes
Recovery
Luminol
Sensors
Limit of Detection
Oxidoreductases

Keywords

  • chemiluminescence reaction
  • fibre-optic biosensor
  • hypoxanthine
  • luminol
  • xanthine

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Fibre-optic biosensor for hypoxanthine and xanthine based on a chemiluminescence reaction. / Hlavay, J.; Haemmerli, S. D.; Guilbault, G. G.

In: Biosensors and Bioelectronics, Vol. 9, No. 3, 1994, p. 189-195.

Research output: Contribution to journalArticle

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