Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol

J. Glogowski, R. Kolman, M. Szczepkowski, Horváth, B. Urbányi, P. Sieczyński, A. Rzemieniecki, J. Domagała, W. Demianowicz, R. Kowalski, A. Ciereszko

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Abstract

Milt obtained from three Siberian sturgeon males (Acipenser baeri, Brandt) were cryopreserved using three extenders: Tris-sucrose-KCl (30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl, pH 8.0), Tris-NaCl (10 mM Tris, 25 mM NaCl, pH 8.5), and Tris-sucrose (20 mM Tris, 400 mM sucrose, pH 8.0) supplemented with 10% methanol. Semen was diluted 1:1 with appropriate extender and frozen in liquid nitrogen vapor. After fertilization with cryopreserved milt, hatching rotes of 29.6±5.0%, 18.2±2.4%, and 6.0±3.0% were recorded for Tris-sucrose-KCl, Tris-NaCl, and Tris-sucrose extender, respectively. Rates for the first two extenders were similar to data of fresh semen obtained from two males(17.9% and 26.0% for male #1 and #2, respectively). Our results indicate that Tris-sucrose-KCl and Tris-NaCl are useful extenders and methanol is a useful cryoprotectant for cryopreservation of sturgeon semen.

Original languageEnglish
Pages (from-to)367-373
Number of pages7
JournalAquaculture
Volume211
Issue number1-4
DOIs
Publication statusPublished - Aug 23 2002

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Keywords

  • Cryopreservation
  • Sperm
  • Sturgeon

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Glogowski, J., Kolman, R., Szczepkowski, M., Horváth, Urbányi, B., Sieczyński, P., Rzemieniecki, A., Domagała, J., Demianowicz, W., Kowalski, R., & Ciereszko, A. (2002). Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol. Aquaculture, 211(1-4), 367-373. https://doi.org/10.1016/S0044-8486(02)00003-0