Fertilization and early embryology

Ongoing pregnancies and birth after intracytoplasmic sperm injection with frozen-thawed epididymal spermatozoa

Paul Devroey, Sherman Silber, Zsolt Nagy, Jiaen Liu, Herman Tournaye, Hubert Joris, Greta Verheyen, Andre Van Steirteghem

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

In seven patients who did not become pregnant following microsurgical epididymal sperm aspiration (MESA) and intracytoplasmic sperm injection (ICSI), a subsequent ICSI was performed using previously cryopreserved supernumerary epididymal spermatozoa without re-operating on the husband. During the original MESA procedure a mean sperm concentration of 12.3×106/ml was achieved. The supernumerary spermatozoa were cryopreserved for later use. After thawing frozen epididymal spermatozoa a mean concentration of 1.9×106 spermatozoa/ml was obtained in straws containing a total volume of sperm suspension of 250 μl. From 68 intact oocytes injected with frozen-thawed epididymal spermatozoa, a two pronuclear fertilization rate of 45% and a cleavage rate of 82% were obtained. A total of 17 embryos were replaced in the seven patients, resulting in two ongoing singleton pregnancies and one twin delivery. Six embryos were cryopreserved. In conclusion, it would appear mandatory to cryopreserve supernumerary spermatozoa during a MESA in order to avoid subsequent further scrotal surgery.

Original languageEnglish
Pages (from-to)903-906
Number of pages4
JournalHuman Reproduction
Volume10
Issue number4
Publication statusPublished - Apr 1995

Fingerprint

Intracytoplasmic Sperm Injection
Spermatozoa
Thawing
Embryology
Fertilization
Intracytoplasmic Sperm Injections
Pregnancy
Straw
Surgery
Parturition
Sperm Retrieval
Embryo
Oocyte
Embryonic Structures
Twin Pregnancy
Sperm
Injection
Spouses
Oocytes
Suspensions

Keywords

  • Fertilization
  • Frozen-thawed epididymal spermatozoa
  • Intracytoplasmic sperm injection
  • Pregnancy

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Rehabilitation
  • Developmental Biology
  • Physiology

Cite this

Fertilization and early embryology : Ongoing pregnancies and birth after intracytoplasmic sperm injection with frozen-thawed epididymal spermatozoa. / Devroey, Paul; Silber, Sherman; Nagy, Zsolt; Liu, Jiaen; Tournaye, Herman; Joris, Hubert; Verheyen, Greta; Van Steirteghem, Andre.

In: Human Reproduction, Vol. 10, No. 4, 04.1995, p. 903-906.

Research output: Contribution to journalArticle

Devroey, P, Silber, S, Nagy, Z, Liu, J, Tournaye, H, Joris, H, Verheyen, G & Van Steirteghem, A 1995, 'Fertilization and early embryology: Ongoing pregnancies and birth after intracytoplasmic sperm injection with frozen-thawed epididymal spermatozoa', Human Reproduction, vol. 10, no. 4, pp. 903-906.
Devroey, Paul ; Silber, Sherman ; Nagy, Zsolt ; Liu, Jiaen ; Tournaye, Herman ; Joris, Hubert ; Verheyen, Greta ; Van Steirteghem, Andre. / Fertilization and early embryology : Ongoing pregnancies and birth after intracytoplasmic sperm injection with frozen-thawed epididymal spermatozoa. In: Human Reproduction. 1995 ; Vol. 10, No. 4. pp. 903-906.
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