Fertilization and early embryology: Maturation of human cumulus-free germinal vesiclestage oocytes to metaphase II by coculture with monolayer vero cells

Cécile Janssenswillen, Zsolt P. Nagy, André Van Steirteghem

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The objective of this study was to evaluate and compare the efficacy of coculture with Vero cells (monkey kidney cells) and in-vitro culture in B2 Ménézo medium for the maturation of germinal vesicle (GV)-stage cumulus-free human oocytes to metaphase II. Cumulus-free human GV-stage oocytes obtained after ovulation induction and retrieval via vaginal ultrasound echo-guided puncture were put either in coculture with Vero cells or in 25 μl droplets of B2 Ménézo medium. In a controlled randomized study, 145 GV-stage oocytes were matured in vitro: 72 were placed in the coculture system with Vero cells and 73 were cultured in B2 alone. After 30 h of in-vitro culture, 82.4% had dissolved the nuclear membrane and extruded the first polar body in the coculture system compared with 37% in B2 medium (P < 0.001). A significant difference was also noticed in the number of GV-stage oocytes which underwent GV breakdown; the proportion of oocytes which did not extrude the first polar body (metaphase I) was 1.6% in the coculture system compared to 44.0% in the B2 medium. It can be concluded that coculture with Vero cells improves the in-vitro maturation of cumulus-free human GV-stage oocytes.

Original languageEnglish
Pages (from-to)375-378
Number of pages4
JournalHuman Reproduction
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 1995

Keywords

  • Coculture
  • Cumulus-free oocytes
  • In-vitro maturation
  • Vero cells

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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