Fermentation characteristics and secretion of proteases of a new keratinolytic strain of Bacillus licheniformis

M. Rozs, L. Manczinger, Cs Vágvölgyi, F. Kevei, A. Hochkoeppler, A. G. Vara Y Rodríguez

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A keratin-degrading strain of Bacillus licheniformis (K-508) was isolated from partially-degraded feathers and characterised. It had high chicken feather-degrading activity when cultured in feather-containing broth, with a growth optimum at pH 7 and 47°C. Broth filtrates were active towards N-Bz-Phe-Val-Arg-p-nitroanilide and N-Suc-Ala-Ala-Pro-Phe-p-nitroanilide, as chromogenic protease substrates at pH 8. Strain K-508 displays keratinolytic activity against native feather keratin (without any pretreatment) in the presence of SH-reducing compounds. It constitutively secreted both trypsin-like and chymotrypsin-like proteases.

Original languageEnglish
Pages (from-to)1925-1929
Number of pages5
JournalBiotechnology Letters
Volume23
Issue number23
DOIs
Publication statusPublished - Dec 27 2001

Keywords

  • Bacillus licheniformis
  • Feathers
  • Keratinase
  • Proteases

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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