Fenugreek (Trigonella Foenum-Graecum) seed flour and diosgenin preserve endothelium-dependent arterial relaxation in a rat model of early-stage metabolic syndrome

Katalin Szabó, Rudolf Gesztelyi, Nóra Lampé, Rita Kiss, Judit Remenyik, Georgina Pesti-Asbóth, Dániel Priksz, Zoltán Szilvássy, Béla Juhász

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Fenugreek is a common herb possessing several bioactive components including diosgenin. Here, dietary fenugreek seed flour and diosgenin were evaluated on a model of endothelium-dependent vasorelaxation by abdominal aortas isolated from rats receiving high-fat, high-sugar diet (HFHSD). 60 male Wistar rats were randomized into six groups: (i) negative control getting conventional rat feed regimen; (ii) positive control receiving HFHSD; (iii) a test group fed 2 g/kg bw/day fenugreek seed flour (containing 10 mg/kg bw/day diosgenin) + HFHSD; (iv) three test groups fed 1, 10 and 50 mg/kg bw/day diosgenin + HFHSD. Alimentary treatments were carried out for six weeks. The abdominal aortas were isolated, and 2 mm wide rings were sectioned off and mounted at a resting tension of 10 mN in organ baths containing Krebs solution (36°C) exposed to 95% O2 and 5% CO2. After 60-min incubation, a norepinephrine concentration-response (E/c) curve was generated to determine their half-maximal effective concentration (EC50) value. After 60-min wash-out, a pre-contraction with norepinephrine EC50 was made, followed by an acetylcholine E/c curve. Plasma glutathione levels, glutathione-handling enzyme activities and blood antioxidant capacities were also determined. HFHSD significantly decreased the dilatory response to acetylcholine and increased plasma glutathione levels and these effects were significantly reversed by fenugreek seed flour, 10 and 50 mg/kg bw/day diosgenin. Both fenugreek and diosgenin treatments prevent HFHSD-induced endothelial dysfunction and redox changes. As fenugreek treatment was more effective at lower acetylcholine concentrations than diosgenin treatments, components of fenugreek other than diosgenin may contribute to the beneficial effects of dietary fenugreek seed flour.

Original languageEnglish
Article number798
JournalInternational Journal of Molecular Sciences
Volume19
Issue number3
DOIs
Publication statusPublished - Mar 10 2018

Fingerprint

Diosgenin
Trigonella
flour
endothelium
diets
fats
Flour
Nutrition
sugars
Oils and fats
Sugars
rats
Endothelium
Seed
Rats
seeds
Seeds
High Fat Diet
acetylcholine
glutathione

Keywords

  • Diosgenin
  • Endothelial dysfunction
  • Endothelium-dependent vasorelaxation
  • Fenugreek
  • Metabolic syndrome
  • Obesity
  • Trigonella foenum-graecum
  • Type 2 diabetes mellitus
  • Wistar rat

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Fenugreek (Trigonella Foenum-Graecum) seed flour and diosgenin preserve endothelium-dependent arterial relaxation in a rat model of early-stage metabolic syndrome. / Szabó, Katalin; Gesztelyi, Rudolf; Lampé, Nóra; Kiss, Rita; Remenyik, Judit; Pesti-Asbóth, Georgina; Priksz, Dániel; Szilvássy, Zoltán; Juhász, Béla.

In: International Journal of Molecular Sciences, Vol. 19, No. 3, 798, 10.03.2018.

Research output: Contribution to journalArticle

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