Feasibility of proton FLASH effect tested by zebrafish embryo irradiation

Elke Beyreuther, Michael Brand, Stefan Hans, K. Hideghéty, Leonhard Karsch, Elisabeth Leßmann, Michael Schürer, Emília Rita Szabó, Jörg Pawelke

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background and purpose: Motivated by first animal trials showing the normal tissue protecting effect of electron and photon Flash irradiation, i.e. at mean dose rates of 100 Gy/s and higher, relative to conventional beam delivery over minutes the feasibility of proton Flash should be assessed. Materials and methods: A setup and beam parameter settings for the treatment of zebrafish embryo with proton Flash and proton beams of conventional dose rate were established at the University Proton Therapy Dresden. Zebrafish embryos were treated with graded doses and the differential effect on embryonic survival and the induction of morphological malformations was followed for up to four days after irradiation. Results: Beam parameters for the realization of proton Flash were set and tested with respect to controlled dose delivery to biological samples. Analyzing the dose dependent embryonic survival and the rate of spinal curvature as one type of developmental abnormality, no significant influence of proton dose rate was revealed. For the rate of pericardial edema as acute radiation effect, a significant difference (p < 0.05) between proton Flash and protons delivered at conventional dose rate of 5 Gy/min was observed for one dose point only. Conclusion: The feasibility of Flash proton irradiation was successfully shown, whereas more experiments are required to confirm the presence or absence of a protecting effect and to figure out the limits and requirements for the Flash effect.

Original languageEnglish
JournalRadiotherapy and Oncology
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Zebrafish
Protons
Embryonic Structures
Embryonic Induction
Spinal Curvatures
Proton Therapy
Radiation Effects
Photons
Edema
Electrons

Keywords

  • Normal tissue toxicity
  • Proton beam therapy
  • Proton Flash effect
  • Zebrafish embryo

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Radiology Nuclear Medicine and imaging

Cite this

Feasibility of proton FLASH effect tested by zebrafish embryo irradiation. / Beyreuther, Elke; Brand, Michael; Hans, Stefan; Hideghéty, K.; Karsch, Leonhard; Leßmann, Elisabeth; Schürer, Michael; Szabó, Emília Rita; Pawelke, Jörg.

In: Radiotherapy and Oncology, 01.01.2019.

Research output: Contribution to journalArticle

Beyreuther, E, Brand, M, Hans, S, Hideghéty, K, Karsch, L, Leßmann, E, Schürer, M, Szabó, ER & Pawelke, J 2019, 'Feasibility of proton FLASH effect tested by zebrafish embryo irradiation', Radiotherapy and Oncology. https://doi.org/10.1016/j.radonc.2019.06.024
Beyreuther, Elke ; Brand, Michael ; Hans, Stefan ; Hideghéty, K. ; Karsch, Leonhard ; Leßmann, Elisabeth ; Schürer, Michael ; Szabó, Emília Rita ; Pawelke, Jörg. / Feasibility of proton FLASH effect tested by zebrafish embryo irradiation. In: Radiotherapy and Oncology. 2019.
@article{a86525d7796b42ddb913238b7338f7bb,
title = "Feasibility of proton FLASH effect tested by zebrafish embryo irradiation",
abstract = "Background and purpose: Motivated by first animal trials showing the normal tissue protecting effect of electron and photon Flash irradiation, i.e. at mean dose rates of 100 Gy/s and higher, relative to conventional beam delivery over minutes the feasibility of proton Flash should be assessed. Materials and methods: A setup and beam parameter settings for the treatment of zebrafish embryo with proton Flash and proton beams of conventional dose rate were established at the University Proton Therapy Dresden. Zebrafish embryos were treated with graded doses and the differential effect on embryonic survival and the induction of morphological malformations was followed for up to four days after irradiation. Results: Beam parameters for the realization of proton Flash were set and tested with respect to controlled dose delivery to biological samples. Analyzing the dose dependent embryonic survival and the rate of spinal curvature as one type of developmental abnormality, no significant influence of proton dose rate was revealed. For the rate of pericardial edema as acute radiation effect, a significant difference (p < 0.05) between proton Flash and protons delivered at conventional dose rate of 5 Gy/min was observed for one dose point only. Conclusion: The feasibility of Flash proton irradiation was successfully shown, whereas more experiments are required to confirm the presence or absence of a protecting effect and to figure out the limits and requirements for the Flash effect.",
keywords = "Normal tissue toxicity, Proton beam therapy, Proton Flash effect, Zebrafish embryo",
author = "Elke Beyreuther and Michael Brand and Stefan Hans and K. Hidegh{\'e}ty and Leonhard Karsch and Elisabeth Le{\ss}mann and Michael Sch{\"u}rer and Szab{\'o}, {Em{\'i}lia Rita} and J{\"o}rg Pawelke",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.radonc.2019.06.024",
language = "English",
journal = "Radiotherapy and Oncology",
issn = "0167-8140",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Feasibility of proton FLASH effect tested by zebrafish embryo irradiation

AU - Beyreuther, Elke

AU - Brand, Michael

AU - Hans, Stefan

AU - Hideghéty, K.

AU - Karsch, Leonhard

AU - Leßmann, Elisabeth

AU - Schürer, Michael

AU - Szabó, Emília Rita

AU - Pawelke, Jörg

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background and purpose: Motivated by first animal trials showing the normal tissue protecting effect of electron and photon Flash irradiation, i.e. at mean dose rates of 100 Gy/s and higher, relative to conventional beam delivery over minutes the feasibility of proton Flash should be assessed. Materials and methods: A setup and beam parameter settings for the treatment of zebrafish embryo with proton Flash and proton beams of conventional dose rate were established at the University Proton Therapy Dresden. Zebrafish embryos were treated with graded doses and the differential effect on embryonic survival and the induction of morphological malformations was followed for up to four days after irradiation. Results: Beam parameters for the realization of proton Flash were set and tested with respect to controlled dose delivery to biological samples. Analyzing the dose dependent embryonic survival and the rate of spinal curvature as one type of developmental abnormality, no significant influence of proton dose rate was revealed. For the rate of pericardial edema as acute radiation effect, a significant difference (p < 0.05) between proton Flash and protons delivered at conventional dose rate of 5 Gy/min was observed for one dose point only. Conclusion: The feasibility of Flash proton irradiation was successfully shown, whereas more experiments are required to confirm the presence or absence of a protecting effect and to figure out the limits and requirements for the Flash effect.

AB - Background and purpose: Motivated by first animal trials showing the normal tissue protecting effect of electron and photon Flash irradiation, i.e. at mean dose rates of 100 Gy/s and higher, relative to conventional beam delivery over minutes the feasibility of proton Flash should be assessed. Materials and methods: A setup and beam parameter settings for the treatment of zebrafish embryo with proton Flash and proton beams of conventional dose rate were established at the University Proton Therapy Dresden. Zebrafish embryos were treated with graded doses and the differential effect on embryonic survival and the induction of morphological malformations was followed for up to four days after irradiation. Results: Beam parameters for the realization of proton Flash were set and tested with respect to controlled dose delivery to biological samples. Analyzing the dose dependent embryonic survival and the rate of spinal curvature as one type of developmental abnormality, no significant influence of proton dose rate was revealed. For the rate of pericardial edema as acute radiation effect, a significant difference (p < 0.05) between proton Flash and protons delivered at conventional dose rate of 5 Gy/min was observed for one dose point only. Conclusion: The feasibility of Flash proton irradiation was successfully shown, whereas more experiments are required to confirm the presence or absence of a protecting effect and to figure out the limits and requirements for the Flash effect.

KW - Normal tissue toxicity

KW - Proton beam therapy

KW - Proton Flash effect

KW - Zebrafish embryo

UR - http://www.scopus.com/inward/record.url?scp=85068051073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068051073&partnerID=8YFLogxK

U2 - 10.1016/j.radonc.2019.06.024

DO - 10.1016/j.radonc.2019.06.024

M3 - Article

AN - SCOPUS:85068051073

JO - Radiotherapy and Oncology

JF - Radiotherapy and Oncology

SN - 0167-8140

ER -