Fast separation of amino acid phenylthiohydantoin derivatives by HPLC on a non-porous stationary phase

B. Boros, K. Kovacs, R. Ohmacht

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A new method is described for the separation of phenylthiohydantoin (PTH) derivatives of the 20 common amino acids. The analysis requires approximately 7 minutes and good resolution is obtained by RP-HPLC on columns packed with a non-porous stationary phase (Kovasil-C14; 33 x 4.6 mm). Gradient elution was chosen with eluents containing either sodium acetate / acetic acid buffers (moderately acidic conditions) or a heptafluorobutyric acid modifier (strongly acidic eluent). A slightly different elution order of the PTH-amino acids was found in the two systems. Low detection limits (in the femtomol range) were achieved with simple commercial HPLC equipment.

Original languageEnglish
JournalChromatographia
Volume51
Issue numberSUPPL. 2
Publication statusPublished - 2000

Fingerprint

Phenylthiohydantoin
High Pressure Liquid Chromatography
Derivatives
Amino Acids
Sodium Acetate
Acetic Acid
Limit of Detection
Buffers
Equipment and Supplies

Keywords

  • Column liquid chromatography
  • Non-porous stationary phases
  • Phenylthiohydantoin (PTH) amino acid separation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Analytical Chemistry

Cite this

Fast separation of amino acid phenylthiohydantoin derivatives by HPLC on a non-porous stationary phase. / Boros, B.; Kovacs, K.; Ohmacht, R.

In: Chromatographia, Vol. 51, No. SUPPL. 2, 2000.

Research output: Contribution to journalArticle

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AU - Ohmacht, R.

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N2 - A new method is described for the separation of phenylthiohydantoin (PTH) derivatives of the 20 common amino acids. The analysis requires approximately 7 minutes and good resolution is obtained by RP-HPLC on columns packed with a non-porous stationary phase (Kovasil-C14; 33 x 4.6 mm). Gradient elution was chosen with eluents containing either sodium acetate / acetic acid buffers (moderately acidic conditions) or a heptafluorobutyric acid modifier (strongly acidic eluent). A slightly different elution order of the PTH-amino acids was found in the two systems. Low detection limits (in the femtomol range) were achieved with simple commercial HPLC equipment.

AB - A new method is described for the separation of phenylthiohydantoin (PTH) derivatives of the 20 common amino acids. The analysis requires approximately 7 minutes and good resolution is obtained by RP-HPLC on columns packed with a non-porous stationary phase (Kovasil-C14; 33 x 4.6 mm). Gradient elution was chosen with eluents containing either sodium acetate / acetic acid buffers (moderately acidic conditions) or a heptafluorobutyric acid modifier (strongly acidic eluent). A slightly different elution order of the PTH-amino acids was found in the two systems. Low detection limits (in the femtomol range) were achieved with simple commercial HPLC equipment.

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