Peptidek gyors HPLC-elválasztása nemporózus állófázison

Translated title of the contribution: Fast HPLC separation of peptides on a nonporous stationary phase

R. Ohmacht, Ibolya Kiss

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A new, 1,5 μm, nonporous, silica based, reversed phase packing (Kovasil-H) was investigated for the peptide mapping of Human and Bovine Serum Albumin, Human and Bovine Insulin and Human and Bovine Cytochrome-c tryptic digests and for the quality control of trypsin and chymotrypsin. Using short columns (33-66 mm) and high temperature, very short analysis times and high sensitivities were attained. No special modification of commonly used liquid chromatographs are necessary for the application of this method.

Original languageHungarian
Pages (from-to)506-510
Number of pages5
JournalMagyar Kemiai Folyoirat, Kemiai Kozlemenyek
Volume103
Issue number10
Publication statusPublished - Oct 1997

Fingerprint

Bovine Serum Albumin
Cytochromes c
Silicon Dioxide
Quality control
Insulin
Peptides
Liquids
Temperature
trypsin drug combination chymotrypsin

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Peptidek gyors HPLC-elválasztása nemporózus állófázison. / Ohmacht, R.; Kiss, Ibolya.

In: Magyar Kemiai Folyoirat, Kemiai Kozlemenyek, Vol. 103, No. 10, 10.1997, p. 506-510.

Research output: Contribution to journalArticle

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