A new, 1,5 μm, nonporous, silica based, reversed phase packing (Kovasil-H) was investigated for the peptide mapping of Human and Bovine Serum Albumin, Human and Bovine Insulin and Human and Bovine Cytochrome-c tryptic digests and for the quality control of trypsin and chymotrypsin. Using short columns (33-66 mm) and high temperature, very short analysis times and high sensitivities were attained. No special modification of commonly used liquid chromatographs are necessary for the application of this method.
|Translated title of the contribution||Fast HPLC separation of peptides on a nonporous stationary phase|
|Number of pages||5|
|Journal||Magyar Kemiai Folyoirat, Kemiai Kozlemenyek|
|Publication status||Published - Oct 1 1997|
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