Fast electric response signals in the bacteriorhodopsin photocycle

P. Ormos, L. Reinisch, L. Keszthelyi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The time behavior of flash-induced charge movements during the first steps in the bacteriorhodopsin photocycle was measured on a suspension of purple membranes oriented by an electric field. The experiments were done in the temperature range 80-278 K. During the formation of the intermediate K, two negative (with respect to the direction of the proton pump) components of the response signal are well resolved with time constants τ1 < 3 μs and τ2 {reversed tilde equals} 150 μs at 200 K. The distances of the charge displacements responsible for the electric signals are estimated. On the basis of the results the two components are assigned to two steps in the trans-cis isomerization of the retinal. A third negative component appears at higher temperatures which is related by time constant measurements to the K → L transition.

Original languageEnglish
Pages (from-to)471-479
Number of pages9
JournalBBA - Bioenergetics
Volume722
Issue number3
DOIs
Publication statusPublished - Mar 18 1983

Keywords

  • Bacteriorhodopsin
  • Photocycle
  • Purple membrane
  • Retinal

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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