The authors report about direct, competitive ELISA tests for the determination of three drug residues (gentamicin, sulphamethazin, sulphadiazine) in milk samples, based on monoclonal antibodies. The assays have been validated by the most important reliability data, e.g. specificity, measuring range, detection limits (Table1, 2, 3 and 4) reproducibility (Table 5 and 6) as well as accuracy (Table 7, 8 and 9). The preparation of milk samples is simple as they are used directly, without any extraction, in the test. The quality parameters of the developed ELISA-tests meet all the EU-requirements relating to the determination of 100 μg/kg residue in milk. Gentamicin measuring range 0.1-10 ng/ml, detection limit of 0.03 ng/ml; Sulphamethazin: measuring range 25-1000 ng/ml, detection limit of 10 ng/ml; Sulphadiazin: measuring range 2.5-50 ng/ml, detection limit of 1.5 ng/ml. Thus, the developed ELISA tests can be used both for preliminary screening and for quantitative determination of drug residues in milk.
|Number of pages||7|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Dec 12 2006|
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