Fast cadmium inhibition of photosynthesis in cyanobacteria in vivo and in vitro studies using perturbed angular correlation of γ-rays

Klára Nárcisz Sas, László Kovács, Ottó Zsíros, Z. Gombos, G. Garab, Lars Hemmingsen, Eva Danielsen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The effect of cadmium on the photosynthetic activity of Synechocystis PCC 6803 was monitored in this study. The oxygen evolving capacity of Synechocystis treated with 40 μM CdCl2 was depressed to 10% of the maximum in 15 min, indicating that Cd2+ penetrated rapidly into the cells and blocked the photosynthetic activity. However, neither photosystem II (PSII) nor photosystem I (PSI) activity showed a significant short-term decrease which would explain this fast decrease in the whole-chain electron transport. Thermoluminescence measurements have shown that the charge separation and stabilization in PSII remains essentially unchanged during the first few hours following the Cd2+ treatment. The electron flow through PSI was monitored by following the redox changes of the P700 reaction centers of PSI. Alterations in the oxidation kinetics of P700 in the Cd2+-treated cells indicated that Cd2+ treatment might affect the available electron acceptor pool of P700, including the CO2 reduction and accumulation in the cells. Perturbed angular correlation of γ-rays (PAC) using the radioactive 111mCd isotope was used to follow the Cd 2+ uptake at a molecular level. The most plausible interpretation of the PAC data is that Cd2+ is taken up by one or more Zn proteins replacing Zn2+ in Synechocystis PCC 6803. Using the radioactive 109Cd isotope, a protein of approximately 30 kDa that binds Cd 2+ could be observed in sodium dodecyl sulfate polyacrylamide gel electrophoresis. The results indicate that Cd2+ might inactivate different metal-containing enzymes, including carbonic anhydrase, by replacing the zinc ion, which would explain the rapid and almost full inhibition of the photosynthetic activity in cyanobacteria.

Original languageEnglish
Pages (from-to)725-734
Number of pages10
JournalJournal of Biological Inorganic Chemistry
Volume11
Issue number6
DOIs
Publication statusPublished - Sep 2006

Fingerprint

Synechocystis
Photosystem I Protein Complex
Photosynthesis
Cyanobacteria
Cadmium
Photosystem II Protein Complex
Radioisotopes
Electrons
Cadmium Chloride
Thermoluminescence
Carbonic Anhydrases
Electron Transport
Electrophoresis
Sodium Dodecyl Sulfate
Oxidation-Reduction
Zinc
Polyacrylamide Gel Electrophoresis
Proteins
Stabilization
Metals

Keywords

  • Cadmium toxicity
  • Cyanobacteria
  • Oxygen-evolving activity
  • Photosynthesis
  • Synechocystis sp. 6803

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Fast cadmium inhibition of photosynthesis in cyanobacteria in vivo and in vitro studies using perturbed angular correlation of γ-rays. / Sas, Klára Nárcisz; Kovács, László; Zsíros, Ottó; Gombos, Z.; Garab, G.; Hemmingsen, Lars; Danielsen, Eva.

In: Journal of Biological Inorganic Chemistry, Vol. 11, No. 6, 09.2006, p. 725-734.

Research output: Contribution to journalArticle

Sas, Klára Nárcisz ; Kovács, László ; Zsíros, Ottó ; Gombos, Z. ; Garab, G. ; Hemmingsen, Lars ; Danielsen, Eva. / Fast cadmium inhibition of photosynthesis in cyanobacteria in vivo and in vitro studies using perturbed angular correlation of γ-rays. In: Journal of Biological Inorganic Chemistry. 2006 ; Vol. 11, No. 6. pp. 725-734.
@article{fd2ed280152040e5b936669c46abe16a,
title = "Fast cadmium inhibition of photosynthesis in cyanobacteria in vivo and in vitro studies using perturbed angular correlation of γ-rays",
abstract = "The effect of cadmium on the photosynthetic activity of Synechocystis PCC 6803 was monitored in this study. The oxygen evolving capacity of Synechocystis treated with 40 μM CdCl2 was depressed to 10{\%} of the maximum in 15 min, indicating that Cd2+ penetrated rapidly into the cells and blocked the photosynthetic activity. However, neither photosystem II (PSII) nor photosystem I (PSI) activity showed a significant short-term decrease which would explain this fast decrease in the whole-chain electron transport. Thermoluminescence measurements have shown that the charge separation and stabilization in PSII remains essentially unchanged during the first few hours following the Cd2+ treatment. The electron flow through PSI was monitored by following the redox changes of the P700 reaction centers of PSI. Alterations in the oxidation kinetics of P700 in the Cd2+-treated cells indicated that Cd2+ treatment might affect the available electron acceptor pool of P700, including the CO2 reduction and accumulation in the cells. Perturbed angular correlation of γ-rays (PAC) using the radioactive 111mCd isotope was used to follow the Cd 2+ uptake at a molecular level. The most plausible interpretation of the PAC data is that Cd2+ is taken up by one or more Zn proteins replacing Zn2+ in Synechocystis PCC 6803. Using the radioactive 109Cd isotope, a protein of approximately 30 kDa that binds Cd 2+ could be observed in sodium dodecyl sulfate polyacrylamide gel electrophoresis. The results indicate that Cd2+ might inactivate different metal-containing enzymes, including carbonic anhydrase, by replacing the zinc ion, which would explain the rapid and almost full inhibition of the photosynthetic activity in cyanobacteria.",
keywords = "Cadmium toxicity, Cyanobacteria, Oxygen-evolving activity, Photosynthesis, Synechocystis sp. 6803",
author = "Sas, {Kl{\'a}ra N{\'a}rcisz} and L{\'a}szl{\'o} Kov{\'a}cs and Ott{\'o} Zs{\'i}ros and Z. Gombos and G. Garab and Lars Hemmingsen and Eva Danielsen",
year = "2006",
month = "9",
doi = "10.1007/s00775-006-0113-x",
language = "English",
volume = "11",
pages = "725--734",
journal = "Journal of Biological Inorganic Chemistry",
issn = "0949-8257",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Fast cadmium inhibition of photosynthesis in cyanobacteria in vivo and in vitro studies using perturbed angular correlation of γ-rays

AU - Sas, Klára Nárcisz

AU - Kovács, László

AU - Zsíros, Ottó

AU - Gombos, Z.

AU - Garab, G.

AU - Hemmingsen, Lars

AU - Danielsen, Eva

PY - 2006/9

Y1 - 2006/9

N2 - The effect of cadmium on the photosynthetic activity of Synechocystis PCC 6803 was monitored in this study. The oxygen evolving capacity of Synechocystis treated with 40 μM CdCl2 was depressed to 10% of the maximum in 15 min, indicating that Cd2+ penetrated rapidly into the cells and blocked the photosynthetic activity. However, neither photosystem II (PSII) nor photosystem I (PSI) activity showed a significant short-term decrease which would explain this fast decrease in the whole-chain electron transport. Thermoluminescence measurements have shown that the charge separation and stabilization in PSII remains essentially unchanged during the first few hours following the Cd2+ treatment. The electron flow through PSI was monitored by following the redox changes of the P700 reaction centers of PSI. Alterations in the oxidation kinetics of P700 in the Cd2+-treated cells indicated that Cd2+ treatment might affect the available electron acceptor pool of P700, including the CO2 reduction and accumulation in the cells. Perturbed angular correlation of γ-rays (PAC) using the radioactive 111mCd isotope was used to follow the Cd 2+ uptake at a molecular level. The most plausible interpretation of the PAC data is that Cd2+ is taken up by one or more Zn proteins replacing Zn2+ in Synechocystis PCC 6803. Using the radioactive 109Cd isotope, a protein of approximately 30 kDa that binds Cd 2+ could be observed in sodium dodecyl sulfate polyacrylamide gel electrophoresis. The results indicate that Cd2+ might inactivate different metal-containing enzymes, including carbonic anhydrase, by replacing the zinc ion, which would explain the rapid and almost full inhibition of the photosynthetic activity in cyanobacteria.

AB - The effect of cadmium on the photosynthetic activity of Synechocystis PCC 6803 was monitored in this study. The oxygen evolving capacity of Synechocystis treated with 40 μM CdCl2 was depressed to 10% of the maximum in 15 min, indicating that Cd2+ penetrated rapidly into the cells and blocked the photosynthetic activity. However, neither photosystem II (PSII) nor photosystem I (PSI) activity showed a significant short-term decrease which would explain this fast decrease in the whole-chain electron transport. Thermoluminescence measurements have shown that the charge separation and stabilization in PSII remains essentially unchanged during the first few hours following the Cd2+ treatment. The electron flow through PSI was monitored by following the redox changes of the P700 reaction centers of PSI. Alterations in the oxidation kinetics of P700 in the Cd2+-treated cells indicated that Cd2+ treatment might affect the available electron acceptor pool of P700, including the CO2 reduction and accumulation in the cells. Perturbed angular correlation of γ-rays (PAC) using the radioactive 111mCd isotope was used to follow the Cd 2+ uptake at a molecular level. The most plausible interpretation of the PAC data is that Cd2+ is taken up by one or more Zn proteins replacing Zn2+ in Synechocystis PCC 6803. Using the radioactive 109Cd isotope, a protein of approximately 30 kDa that binds Cd 2+ could be observed in sodium dodecyl sulfate polyacrylamide gel electrophoresis. The results indicate that Cd2+ might inactivate different metal-containing enzymes, including carbonic anhydrase, by replacing the zinc ion, which would explain the rapid and almost full inhibition of the photosynthetic activity in cyanobacteria.

KW - Cadmium toxicity

KW - Cyanobacteria

KW - Oxygen-evolving activity

KW - Photosynthesis

KW - Synechocystis sp. 6803

UR - http://www.scopus.com/inward/record.url?scp=33746218417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746218417&partnerID=8YFLogxK

U2 - 10.1007/s00775-006-0113-x

DO - 10.1007/s00775-006-0113-x

M3 - Article

C2 - 16821039

AN - SCOPUS:33746218417

VL - 11

SP - 725

EP - 734

JO - Journal of Biological Inorganic Chemistry

JF - Journal of Biological Inorganic Chemistry

SN - 0949-8257

IS - 6

ER -