Far-red fluorescence: A direct spectroscopic marker for LHCII oligomer formation in non-photochemical quenching

Y. Miloslavina, Antje Wehner, P. Lambrev, Emilie Wientjes, Michael Reus, G. Garab, Roberta Croce, Alfred R. Holzwarth

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

Time-resolved fluorescence on oligomers of the main light-harvesting complex from higher plants indicate that in vitro oligomerization leads to the formation of a weakly coupled inter-trimer chlorophyll-chlorophyll (Chl) exciton state which converts in tens of ps into a state which is spectrally broad and has a strongly far-red enhanced fluorescence spectrum. Both its lifetime and spectrum show striking similarity with a 400 ps fluorescence component appearing in intact leaves of Arabidopsis when non-photochemical quenching (NPQ) is induced. The fluorescence components with high far-red/red ratio are thus a characteristic marker for NPQ conditions in vivo. The far-red emitting state is shown to be an emissive Chl-Chl charge transfer state which plays a crucial part in the quenching.

Original languageEnglish
Pages (from-to)3625-3631
Number of pages7
JournalFEBS Letters
Volume582
Issue number25-26
DOIs
Publication statusPublished - Oct 29 2008

Fingerprint

Chlorophyll
Oligomers
Quenching
Fluorescence
Oligomerization
Arabidopsis
Charge transfer
Light

Keywords

  • Aggregation
  • Arabidopsis mutant
  • Chlorophyll fluorescence
  • LHCII
  • Non-photochemical quenching
  • Violaxanthin
  • Zeaxanthin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Far-red fluorescence : A direct spectroscopic marker for LHCII oligomer formation in non-photochemical quenching. / Miloslavina, Y.; Wehner, Antje; Lambrev, P.; Wientjes, Emilie; Reus, Michael; Garab, G.; Croce, Roberta; Holzwarth, Alfred R.

In: FEBS Letters, Vol. 582, No. 25-26, 29.10.2008, p. 3625-3631.

Research output: Contribution to journalArticle

Miloslavina, Y. ; Wehner, Antje ; Lambrev, P. ; Wientjes, Emilie ; Reus, Michael ; Garab, G. ; Croce, Roberta ; Holzwarth, Alfred R. / Far-red fluorescence : A direct spectroscopic marker for LHCII oligomer formation in non-photochemical quenching. In: FEBS Letters. 2008 ; Vol. 582, No. 25-26. pp. 3625-3631.
@article{1c67be87728a493e841d3dba8f5d283a,
title = "Far-red fluorescence: A direct spectroscopic marker for LHCII oligomer formation in non-photochemical quenching",
abstract = "Time-resolved fluorescence on oligomers of the main light-harvesting complex from higher plants indicate that in vitro oligomerization leads to the formation of a weakly coupled inter-trimer chlorophyll-chlorophyll (Chl) exciton state which converts in tens of ps into a state which is spectrally broad and has a strongly far-red enhanced fluorescence spectrum. Both its lifetime and spectrum show striking similarity with a 400 ps fluorescence component appearing in intact leaves of Arabidopsis when non-photochemical quenching (NPQ) is induced. The fluorescence components with high far-red/red ratio are thus a characteristic marker for NPQ conditions in vivo. The far-red emitting state is shown to be an emissive Chl-Chl charge transfer state which plays a crucial part in the quenching.",
keywords = "Aggregation, Arabidopsis mutant, Chlorophyll fluorescence, LHCII, Non-photochemical quenching, Violaxanthin, Zeaxanthin",
author = "Y. Miloslavina and Antje Wehner and P. Lambrev and Emilie Wientjes and Michael Reus and G. Garab and Roberta Croce and Holzwarth, {Alfred R.}",
year = "2008",
month = "10",
day = "29",
doi = "10.1016/j.febslet.2008.09.044",
language = "English",
volume = "582",
pages = "3625--3631",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "25-26",

}

TY - JOUR

T1 - Far-red fluorescence

T2 - A direct spectroscopic marker for LHCII oligomer formation in non-photochemical quenching

AU - Miloslavina, Y.

AU - Wehner, Antje

AU - Lambrev, P.

AU - Wientjes, Emilie

AU - Reus, Michael

AU - Garab, G.

AU - Croce, Roberta

AU - Holzwarth, Alfred R.

PY - 2008/10/29

Y1 - 2008/10/29

N2 - Time-resolved fluorescence on oligomers of the main light-harvesting complex from higher plants indicate that in vitro oligomerization leads to the formation of a weakly coupled inter-trimer chlorophyll-chlorophyll (Chl) exciton state which converts in tens of ps into a state which is spectrally broad and has a strongly far-red enhanced fluorescence spectrum. Both its lifetime and spectrum show striking similarity with a 400 ps fluorescence component appearing in intact leaves of Arabidopsis when non-photochemical quenching (NPQ) is induced. The fluorescence components with high far-red/red ratio are thus a characteristic marker for NPQ conditions in vivo. The far-red emitting state is shown to be an emissive Chl-Chl charge transfer state which plays a crucial part in the quenching.

AB - Time-resolved fluorescence on oligomers of the main light-harvesting complex from higher plants indicate that in vitro oligomerization leads to the formation of a weakly coupled inter-trimer chlorophyll-chlorophyll (Chl) exciton state which converts in tens of ps into a state which is spectrally broad and has a strongly far-red enhanced fluorescence spectrum. Both its lifetime and spectrum show striking similarity with a 400 ps fluorescence component appearing in intact leaves of Arabidopsis when non-photochemical quenching (NPQ) is induced. The fluorescence components with high far-red/red ratio are thus a characteristic marker for NPQ conditions in vivo. The far-red emitting state is shown to be an emissive Chl-Chl charge transfer state which plays a crucial part in the quenching.

KW - Aggregation

KW - Arabidopsis mutant

KW - Chlorophyll fluorescence

KW - LHCII

KW - Non-photochemical quenching

KW - Violaxanthin

KW - Zeaxanthin

UR - http://www.scopus.com/inward/record.url?scp=53949088888&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53949088888&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2008.09.044

DO - 10.1016/j.febslet.2008.09.044

M3 - Article

C2 - 18834884

AN - SCOPUS:53949088888

VL - 582

SP - 3625

EP - 3631

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 25-26

ER -