Anti-human immundeficiencia vírus (HIV) álpozitivitás vizsgálata krónikus haemodialysissel kezelt betegekben.

Translated title of the contribution: False positive results of HIV virus tests in patients undergoing chronic hemodialysis

E. Újhelyi, G. Gál, J. Makó, G. Füst, B. Büki, K. Nagy, D. T. Ferenc, M. P. Dietrich, P. Hengster, V. Mayer

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The sera of 173 haemodialysis patients treated in two dialysis centers in Hungary were tested for the presence of HIV (HTLV III/LAV) antibodies. Four different commercial enzyme immunoassay (EIA) kits and two types (CEM/LAV, and H9/HTLV III) of indirect immunofluorescence assay (IFA) were used. The Western blot technique was applied as confirmatory test in the study. No confirmed positive results were found in any of the cases. However, in 15 patients (8.7%) false positive (not confirmable by the Western blot assay) results were obtained in at least one but mostly in all of the three type 1 EIA kits (ORGANON, ELECTRONUCLEONICS, SORIN) applied. In 4 patients, the IFA assay also gave false positive results which could be repeated in sequential samples taken from the same patients. Increased reactivity in the control plate (coated with a concentrate of cellular material shed by uninfected H9 cell line) of the SORIN kit was found only in a few false positive samples and no fluorescence with the uninfected H9 or CEM cells was observed in any of the sera showing a false positive IFA. These results indicate that the false positive anti-HIV results frequently observable in haemodialysis patients are not simply the consequence of the presence of antibodies reacting with the uninfected H9 and/or CEM cells but they are most probably due to antibodies against antigens expressed on these cells only after infection with the human immunodeficiency virus.

Original languageHungarian
Pages (from-to)67-70
Number of pages4
JournalOrvosi Hetilap
Volume130
Issue number2
Publication statusPublished - Jan 8 1989

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Renal Dialysis
HIV
Viruses
Immunoenzyme Techniques
Fluorescent Antibody Technique
Western Blotting
HIV Antibodies
Hungary
Antibodies
Indirect Fluorescent Antibody Technique
Serum
Dialysis
Fluorescence
Antigens
Cell Line
Infection

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Anti-human immundeficiencia vírus (HIV) álpozitivitás vizsgálata krónikus haemodialysissel kezelt betegekben. / Újhelyi, E.; Gál, G.; Makó, J.; Füst, G.; Büki, B.; Nagy, K.; Ferenc, D. T.; Dietrich, M. P.; Hengster, P.; Mayer, V.

In: Orvosi Hetilap, Vol. 130, No. 2, 08.01.1989, p. 67-70.

Research output: Contribution to journalArticle

Újhelyi, E, Gál, G, Makó, J, Füst, G, Büki, B, Nagy, K, Ferenc, DT, Dietrich, MP, Hengster, P & Mayer, V 1989, 'Anti-human immundeficiencia vírus (HIV) álpozitivitás vizsgálata krónikus haemodialysissel kezelt betegekben.', Orvosi Hetilap, vol. 130, no. 2, pp. 67-70.
Újhelyi, E. ; Gál, G. ; Makó, J. ; Füst, G. ; Büki, B. ; Nagy, K. ; Ferenc, D. T. ; Dietrich, M. P. ; Hengster, P. ; Mayer, V. / Anti-human immundeficiencia vírus (HIV) álpozitivitás vizsgálata krónikus haemodialysissel kezelt betegekben. In: Orvosi Hetilap. 1989 ; Vol. 130, No. 2. pp. 67-70.
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