Failure in the compensatory mechanism in red blood cells due to sustained smoking during pregnancy

Payal Chakraborty, Krisztina N. Dugmonits, Attila G. Végh, Réka Hollandi, P. Horváth, József Maléth, P. Hegyi, Gábor Németh, E. Hermesz

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Decrease in the bioavailability of vasoactive nitric oxide (NO), derived from the endothelial nitric oxide synthase (NOS3), underlines vascular endothelial damage. Our expanding knowledge on mature red blood cells (RBCs) makes it supposable that RBCs might contribute to vascular function and integrity via their active NO synthetizing system (RBC-NOS3). This “rescue” mechanism of RBCs could be especially important during pregnancy with smoking habit, when smoking acts as an additional stressor and causes active change in the redox status. In this study RBC populations of 82 non-smoking (RBC-NS) and 75 smoking (RBC-S) pregnant women were examined. Morphological variants were followed by confocal microscopy and quantified by a microscopy based intelligent analysis software. Fluorescence activated cell sorting was used to examine the translational and posttranslational regulation of RBC-NOS, Arginase-1 and the formation of the major product of lipid peroxidation, 4-hydroxy-2-nonenal. To survey the rheological parameters of RBCs like elasticity and plasticity atomic force microscopy-based measurement was applied. Significant morphological and functional differences of RBCs were found between the non-smoking and smoking groups. The phenotypic variations in RBC-S population, even the characteristic biconcave disc-shaped cells, could be connected to impaired NOS3 activation and are compromised in their physiological properties. Membrane lipid studies reveal an elevated lipid oxidation state well paralleled with the changed elastic and plastic activities. These features can form a basic tool in the prenatal health screening conditions; hence the compensatory mechanism of RBC-S population completely fails to sense and rescue the acute oxidative stress conditions.

Original languageEnglish
Article number108821
JournalChemico-Biological Interactions
Volume313
DOIs
Publication statusPublished - Nov 1 2019

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Blood
Erythrocytes
Smoking
Cells
Pregnancy
Blood Vessels
Nitric Oxide
Lipids
Arginase
Oxidative stress
Confocal microscopy
Nitric Oxide Synthase Type III
Atomic Force Microscopy
Elasticity
Population Characteristics
Membrane Lipids
Prenatal Diagnosis
Sorting
Confocal Microscopy
Lipid Peroxidation

ASJC Scopus subject areas

  • Toxicology

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Failure in the compensatory mechanism in red blood cells due to sustained smoking during pregnancy. / Chakraborty, Payal; Dugmonits, Krisztina N.; Végh, Attila G.; Hollandi, Réka; Horváth, P.; Maléth, József; Hegyi, P.; Németh, Gábor; Hermesz, E.

In: Chemico-Biological Interactions, Vol. 313, 108821, 01.11.2019.

Research output: Contribution to journalArticle

Chakraborty, Payal ; Dugmonits, Krisztina N. ; Végh, Attila G. ; Hollandi, Réka ; Horváth, P. ; Maléth, József ; Hegyi, P. ; Németh, Gábor ; Hermesz, E. / Failure in the compensatory mechanism in red blood cells due to sustained smoking during pregnancy. In: Chemico-Biological Interactions. 2019 ; Vol. 313.
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