FAD Transport and FAD-dependent Protein Thiol Oxidation in Rat Liver Microsomes

Marianne Varsányi, A. Szarka, Eszter Papp, Dóra Makai, G. Nardai, Rosella Fulceri, P. Csermely, J. Mandl, Angelo Benedetti, G. Bánhegyi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The transport of FAD and its effect on disulfide bond formation was investigated in rat liver microsomal vesicles. By measuring the intravesicular FAD-accessible space, we observed that FAD permeates across the microsomal membrane and accumulates in the lumen. Rapid filtration experiments also demonstrated the uptake and efflux of the compound, which could be inhibited by atractyloside and 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid. FAD entering the lumen promoted the oxidation of protein thiols and increased the intraluminal oxidation of glucose-6-phosphate. These findings support the notion that, similar to yeast, free FAD may have a decisive role in the mechanism of oxidative protein folding in the endoplasmic reticulum lumen of mammalian cells.

Original languageEnglish
Pages (from-to)3370-3374
Number of pages5
JournalJournal of Biological Chemistry
Volume279
Issue number5
DOIs
Publication statusPublished - Jan 30 2004

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Flavin-Adenine Dinucleotide
Liver Microsomes
Sulfhydryl Compounds
Liver
Rats
Oxidation
Proteins
Atractyloside
Protein folding
Glucose-6-Phosphate
Protein Folding
Endoplasmic Reticulum
Disulfides
Yeast
Yeasts
Cells
Membranes
Acids
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

FAD Transport and FAD-dependent Protein Thiol Oxidation in Rat Liver Microsomes. / Varsányi, Marianne; Szarka, A.; Papp, Eszter; Makai, Dóra; Nardai, G.; Fulceri, Rosella; Csermely, P.; Mandl, J.; Benedetti, Angelo; Bánhegyi, G.

In: Journal of Biological Chemistry, Vol. 279, No. 5, 30.01.2004, p. 3370-3374.

Research output: Contribution to journalArticle

Varsányi, Marianne ; Szarka, A. ; Papp, Eszter ; Makai, Dóra ; Nardai, G. ; Fulceri, Rosella ; Csermely, P. ; Mandl, J. ; Benedetti, Angelo ; Bánhegyi, G. / FAD Transport and FAD-dependent Protein Thiol Oxidation in Rat Liver Microsomes. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 5. pp. 3370-3374.
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