Facts and myths about columns packed with sub-3 μm and sub-2 μm particles

Szabolcs Fekete, Katalin Ganzler, J. Fekete

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Increasing the separating efficiency enhances the separation power. The most popular solution for improving chromatographic performance is to employ columns packed with small particle diameters (i.e., sub-2 μm particles) to induce a simultaneous improvement in efficiency, optimal velocity and mass transfer, albeit the cost of pressure. In this study a systematic evaluation of the possibilities and limitations of the separations obtained with 5 cm long narrow bore columns packed with 1.5-3.0 μm particles is presented. Several commercially available different sub-3 μm and sub-2 μm packed columns were evaluated by using van Deemter, Knox and kinetic plots. Theoretical Poppe plots were constructed for each column to compare their kinetic performance. Data are presented on different polar neutral real life analytes, to show that the separation time is not obviously shorter if the particle size is reduced. Comparison of low-molecular weight compounds (one steroid and one non-steroid hormone, with molecular weights lower than 500) and a high-molecular weight one (MW ∼ 1000) was conducted. Same efficiency can be achieved with columns packed with 1.9-2.1 μm particles as with smaller particles. The column packed with 3 μm particles had the lowest reduced plate height minimum (h = 2.2) while the column with the smallest particles (1.5 μm) gave the highest reduced plate height minimum (h ∼ 3.0). According to this study, the theoretically expected efficiency of very fine particles (diameter

Original languageEnglish
Pages (from-to)56-64
Number of pages9
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume51
Issue number1
DOIs
Publication statusPublished - Jan 5 2010

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Molecular Weight
Molecular weight
Particle Size
Kinetics
Steroids
Hormones
Pressure
Costs and Cost Analysis
Mass transfer
Particle size
Costs

Keywords

  • Fast chromatography
  • Kinetic efficiency
  • Low- and high-molecular weight test compounds
  • Poppe plot
  • Sub-2 micron particles

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry

Cite this

Facts and myths about columns packed with sub-3 μm and sub-2 μm particles. / Fekete, Szabolcs; Ganzler, Katalin; Fekete, J.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 51, No. 1, 05.01.2010, p. 56-64.

Research output: Contribution to journalArticle

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