Factors influencing the retention of insulins in reversed-phase high-performance liquid chromatographic systems

Gyula Vigh, Zita Varga-Puchony, J. Hlavay, E. Papp-Hites

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The effects of the concentrations of methanol, buffer and neutral salt, of pH and temperature, as well as of the type, chain length and concentration of ion-pair reagents added to aqueous phosphoate buffer-methanol eluents upon the retention of bovine and porcine have been investigated in detail. A possible explanation for the retention-influencing effects of both the cationic and anionic ion-pair reagents is proposed.

Original languageEnglish
Pages (from-to)51-59
Number of pages9
JournalJournal of Chromatography A
Volume236
Issue number1
DOIs
Publication statusPublished - Feb 5 1982

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Insulins
Methanol
Buffers
Ions
Liquids
Chain length
Swine
Salts
Temperature

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Factors influencing the retention of insulins in reversed-phase high-performance liquid chromatographic systems. / Vigh, Gyula; Varga-Puchony, Zita; Hlavay, J.; Papp-Hites, E.

In: Journal of Chromatography A, Vol. 236, No. 1, 05.02.1982, p. 51-59.

Research output: Contribution to journalArticle

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