Factors influencing the immobilization of glucoamylase

D. Gombin, G. Klamár, B. Szajáni

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Glucoamylase produced by Aspergillus niger was covalently attached to a polyacrylamide bead support possessing carboxylic functional groups activated by water-soluble carbodiimides. Factors influencing the immobilization were studied. The most favourable carbodiimide for the immobilization was N-t-butyl-N′-dimethylaminopropyl carbodiimide methyl iodide. In the experiments in which N-cyclohexyl-N′-morpholinoethyl carbodiimde methyl tosylate was used as coupling agent, the optimum medium was 0.1 M potassium phosphate buffer (pII 7.5). The support can be saturated with protein. In accordance with the molecular weight of the glucoamylase, supports with an exclusion limit of 100,000 daltons or more proved to be most advantageous.

Original languageEnglish
Pages (from-to)9-13
Number of pages5
JournalActa Biologica Szegediensis
Volume41
Issue number1
Publication statusPublished - Dec 1 1997

Keywords

  • Aspergillus niger
  • Carbodiimide effects
  • Covalent bonds
  • Glucoamylase
  • Immobilization, polyacrilamide support
  • Medium
  • Support porosity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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