Factors contributing to enhanced freezing tolerance in wheat during frost hardening in the light

T. Janda, G. Szalai, Kornélia Leskó, Rusina Yordanova, Simona Apostol, Losanka Petrova Popova

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The interaction between light and temperature during the development of freezing tolerance was studied in winter wheat (Triticum aestivum L. var. Mv Emese). Ten-day-old plants were cold hardened at 5 °C for 12 days under normal (250 μmol m-2 s-1) or low light (20 μmol m-2 s-1) conditions. Some of the plants were kept at 20/18 °C for 12 days at high light intensity (500 μmol m-2 s-1), which also increased the freezing tolerance of winter wheat. The freezing survival rate, the lipid composition, the antioxidant activity, and the salicylic acid content were investigated during frost hardening. The saturation level of hexadecanoic acid decreased not only in plants hardened at low temperature, but also, to a lesser extent, in plants kept under high light irradiation at normal growth temperature. The greatest induction of the enzymes glutathione reductase (EC 1.6.4.2.) and ascorbate peroxidase (EC 1.11.1.11.) occurred when the cold treatment was carried out in normal light, but high light intensity at normal, non-hardening temperature also increased the activity of these enzymes. The catalase (EC 1.11.1.6.) activity was also higher in plants grown at high light intensity than in the controls. The greatest level of induction in the activity of the guaiacol peroxidase (EC 1.11.1.7.) enzyme occurred under cold conditions with low light. The bound ortho-hydroxy-cinnamic acid increased by up to two orders of magnitude in plants that were cold hardened in normal light. Both high light intensity and low temperature hardening caused an increase in the free and bound salicylic acid content of the leaves. This increase was most pronounced in plants that were cold treated in normal light.

Original languageEnglish
Pages (from-to)1674-1682
Number of pages9
JournalPhytochemistry
Volume68
Issue number12
DOIs
Publication statusPublished - Jun 2007

Fingerprint

frost
cold tolerance
Freezing
Triticum
Hardening
High intensity light
Light
wheat
light intensity
Salicylic Acid
temperature
glutathione-disulfide reductase
ascorbate peroxidase
salicylic acid
Temperature
winter wheat
catalase
peroxidase
Enzymes
Ascorbate Peroxidases

Keywords

  • Antioxidant enzymes
  • Fatty acid composition
  • Frost tolerance
  • Salicylic acid
  • Triticum aestivum L.

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery

Cite this

Factors contributing to enhanced freezing tolerance in wheat during frost hardening in the light. / Janda, T.; Szalai, G.; Leskó, Kornélia; Yordanova, Rusina; Apostol, Simona; Popova, Losanka Petrova.

In: Phytochemistry, Vol. 68, No. 12, 06.2007, p. 1674-1682.

Research output: Contribution to journalArticle

Janda, T. ; Szalai, G. ; Leskó, Kornélia ; Yordanova, Rusina ; Apostol, Simona ; Popova, Losanka Petrova. / Factors contributing to enhanced freezing tolerance in wheat during frost hardening in the light. In: Phytochemistry. 2007 ; Vol. 68, No. 12. pp. 1674-1682.
@article{04a9cc41c0ad40ca94ff5556486c33c1,
title = "Factors contributing to enhanced freezing tolerance in wheat during frost hardening in the light",
abstract = "The interaction between light and temperature during the development of freezing tolerance was studied in winter wheat (Triticum aestivum L. var. Mv Emese). Ten-day-old plants were cold hardened at 5 °C for 12 days under normal (250 μmol m-2 s-1) or low light (20 μmol m-2 s-1) conditions. Some of the plants were kept at 20/18 °C for 12 days at high light intensity (500 μmol m-2 s-1), which also increased the freezing tolerance of winter wheat. The freezing survival rate, the lipid composition, the antioxidant activity, and the salicylic acid content were investigated during frost hardening. The saturation level of hexadecanoic acid decreased not only in plants hardened at low temperature, but also, to a lesser extent, in plants kept under high light irradiation at normal growth temperature. The greatest induction of the enzymes glutathione reductase (EC 1.6.4.2.) and ascorbate peroxidase (EC 1.11.1.11.) occurred when the cold treatment was carried out in normal light, but high light intensity at normal, non-hardening temperature also increased the activity of these enzymes. The catalase (EC 1.11.1.6.) activity was also higher in plants grown at high light intensity than in the controls. The greatest level of induction in the activity of the guaiacol peroxidase (EC 1.11.1.7.) enzyme occurred under cold conditions with low light. The bound ortho-hydroxy-cinnamic acid increased by up to two orders of magnitude in plants that were cold hardened in normal light. Both high light intensity and low temperature hardening caused an increase in the free and bound salicylic acid content of the leaves. This increase was most pronounced in plants that were cold treated in normal light.",
keywords = "Antioxidant enzymes, Fatty acid composition, Frost tolerance, Salicylic acid, Triticum aestivum L.",
author = "T. Janda and G. Szalai and Korn{\'e}lia Lesk{\'o} and Rusina Yordanova and Simona Apostol and Popova, {Losanka Petrova}",
year = "2007",
month = "6",
doi = "10.1016/j.phytochem.2007.04.012",
language = "English",
volume = "68",
pages = "1674--1682",
journal = "Phytochemistry",
issn = "0031-9422",
publisher = "Elsevier Limited",
number = "12",

}

TY - JOUR

T1 - Factors contributing to enhanced freezing tolerance in wheat during frost hardening in the light

AU - Janda, T.

AU - Szalai, G.

AU - Leskó, Kornélia

AU - Yordanova, Rusina

AU - Apostol, Simona

AU - Popova, Losanka Petrova

PY - 2007/6

Y1 - 2007/6

N2 - The interaction between light and temperature during the development of freezing tolerance was studied in winter wheat (Triticum aestivum L. var. Mv Emese). Ten-day-old plants were cold hardened at 5 °C for 12 days under normal (250 μmol m-2 s-1) or low light (20 μmol m-2 s-1) conditions. Some of the plants were kept at 20/18 °C for 12 days at high light intensity (500 μmol m-2 s-1), which also increased the freezing tolerance of winter wheat. The freezing survival rate, the lipid composition, the antioxidant activity, and the salicylic acid content were investigated during frost hardening. The saturation level of hexadecanoic acid decreased not only in plants hardened at low temperature, but also, to a lesser extent, in plants kept under high light irradiation at normal growth temperature. The greatest induction of the enzymes glutathione reductase (EC 1.6.4.2.) and ascorbate peroxidase (EC 1.11.1.11.) occurred when the cold treatment was carried out in normal light, but high light intensity at normal, non-hardening temperature also increased the activity of these enzymes. The catalase (EC 1.11.1.6.) activity was also higher in plants grown at high light intensity than in the controls. The greatest level of induction in the activity of the guaiacol peroxidase (EC 1.11.1.7.) enzyme occurred under cold conditions with low light. The bound ortho-hydroxy-cinnamic acid increased by up to two orders of magnitude in plants that were cold hardened in normal light. Both high light intensity and low temperature hardening caused an increase in the free and bound salicylic acid content of the leaves. This increase was most pronounced in plants that were cold treated in normal light.

AB - The interaction between light and temperature during the development of freezing tolerance was studied in winter wheat (Triticum aestivum L. var. Mv Emese). Ten-day-old plants were cold hardened at 5 °C for 12 days under normal (250 μmol m-2 s-1) or low light (20 μmol m-2 s-1) conditions. Some of the plants were kept at 20/18 °C for 12 days at high light intensity (500 μmol m-2 s-1), which also increased the freezing tolerance of winter wheat. The freezing survival rate, the lipid composition, the antioxidant activity, and the salicylic acid content were investigated during frost hardening. The saturation level of hexadecanoic acid decreased not only in plants hardened at low temperature, but also, to a lesser extent, in plants kept under high light irradiation at normal growth temperature. The greatest induction of the enzymes glutathione reductase (EC 1.6.4.2.) and ascorbate peroxidase (EC 1.11.1.11.) occurred when the cold treatment was carried out in normal light, but high light intensity at normal, non-hardening temperature also increased the activity of these enzymes. The catalase (EC 1.11.1.6.) activity was also higher in plants grown at high light intensity than in the controls. The greatest level of induction in the activity of the guaiacol peroxidase (EC 1.11.1.7.) enzyme occurred under cold conditions with low light. The bound ortho-hydroxy-cinnamic acid increased by up to two orders of magnitude in plants that were cold hardened in normal light. Both high light intensity and low temperature hardening caused an increase in the free and bound salicylic acid content of the leaves. This increase was most pronounced in plants that were cold treated in normal light.

KW - Antioxidant enzymes

KW - Fatty acid composition

KW - Frost tolerance

KW - Salicylic acid

KW - Triticum aestivum L.

UR - http://www.scopus.com/inward/record.url?scp=34249978455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249978455&partnerID=8YFLogxK

U2 - 10.1016/j.phytochem.2007.04.012

DO - 10.1016/j.phytochem.2007.04.012

M3 - Article

C2 - 17537468

AN - SCOPUS:34249978455

VL - 68

SP - 1674

EP - 1682

JO - Phytochemistry

JF - Phytochemistry

SN - 0031-9422

IS - 12

ER -